玫瑰木苷是中泽 extract 中的一种具有体外抗丙型肝炎病毒活性的生物活性化合物。
Roseoside Is a Bioactive Compound in Nakai Extract with Potent In Vitro Antiviral Activity Against Hepatitis C Virus.
机构信息
Department of Life Science, Gachon University, Seongnam 13120, Republic of Korea.
Natural Product Informatics Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Gangneung 25451, Republic of Korea.
出版信息
Molecules. 2024 Oct 30;29(21):5130. doi: 10.3390/molecules29215130.
Hepatitis C virus (HCV) is a pathogen that causes cirrhosis and hepatocellular carcinoma through chronic hepatitis C. This study focused on the anti-HCV activity of a 70% ethanol extract of Nakai (KKE) and its bioactive chemical constituent(s). The KKE and its -butanol (-BuOH) fraction induced a significant reduction in HCV RNA levels without inducing cytotoxicity. A high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis revealed the presence of roseoside in the -butanol fraction of the KKE, which inhibited HCV RNA replication in a concentration- and time-dependent manner without exerting cytotoxicity. Consistent with in silico molecular docking analysis data, roseoside targets and inhibits HCV NS5A/B replicase. Collectively, our findings demonstrate that roseoside is a chemical constituent in KKE that interferes with HCV replication by targeting NS5A/B replicase.
丙型肝炎病毒（HCV）是一种病原体，可通过慢性丙型肝炎导致肝硬化和肝细胞癌。本研究主要关注的是 Nakai（KKE）的 70%乙醇提取物及其生物活性化学成分对 HCV 的抗病毒活性。KKE 及其正丁醇（-BuOH）级分可在不诱导细胞毒性的情况下显著降低 HCV RNA 水平。高效液相色谱-质谱（HPLC-MS）分析表明，KKE 的正丁醇级分中存在玫瑰糖苷，它以浓度和时间依赖的方式抑制 HCV RNA 复制，同时不产生细胞毒性。与计算机分子对接分析数据一致，玫瑰糖苷靶向并抑制 HCV NS5A/B 复制酶。综上所述，我们的研究结果表明，玫瑰糖苷是 KKE 的一种化学成分，通过靶向 NS5A/B 复制酶干扰 HCV 复制。
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