Microbiology Group, Laboratory of Cellular and Molecular Biology, Faculty of Biological Sciences, University of Science and Technology-Houari Boumediene, 16111 Bab Ezzouar, Algiers, Algeria.
Centre Hospitalier Universitaire Mustapha Bacha, Service de Microbiologie, 16000 Alger, Algérie.
Lett Appl Microbiol. 2024 Nov 6;77(11). doi: 10.1093/lambio/ovae108.
The objective of this study was to characterize Staphylococcus aureus isolates recovered from the nasal samples of healthy pet cats in Algiers province. A total of 138 nasal swabs were collected. Antimicrobial susceptibility was conducted using the disk-diffusion method and the VITEK-2 susceptibility system. Whole genome sequencing was performed to identify multiple-locus sequence typing, antimicrobial and virulence genes. Staphylococcus aureus isolates were detected in 23 cats. Among these, 11 were methicillin-resistant S. aureus (MRSA) (one isolate/sample). Three sequence types (ST6, ST5, and ST1) were identified in MRSA, with the predominance of ST6 (n = 7). Seven distinct STs [ST398, ST97, ST15, ST7, ST291, ST5043, and a new ST, (ST9219)] were detected in methicillin-sensitive S. aureus. All MRSA isolates harbored the mecA gene and SCCmec-type-IVa. MRSA exhibited resistance to tetracycline [n = 3/tet(L) and tet(M); n = 1/tet(K)], kanamycin-tobramycin [n = 3/ant(4')-Ia), amikacin-kanamycin (n = 1/aph(3')-IIIa], and erythromycin-clindamycin [n = 1/erm(C)]. Seven S. aureus isolates were multidrug resistant. All the isolates were negative for lukS/lukF-PV and tst-1 genes, while 20 isolates were IEC-positive. This study revealed a diversity of genetic lineages in S. aureus strains isolated from nasal samples of pet cats, including multidrug-resistant and toxigenic strains. The presence of IEC-positive S. aureus suggests possible human-animal transmission.
本研究旨在分析阿尔及利亚省健康宠物猫鼻腔样本中分离的金黄色葡萄球菌。共采集了 138 份鼻腔拭子。采用纸片扩散法和 VITEK-2 药敏系统进行药敏试验。对全基因组进行测序以确定多位点序列分型、抗菌和毒力基因。在 23 只猫中检测到金黄色葡萄球菌。其中，11 株为耐甲氧西林金黄色葡萄球菌（MRSA）（每株/份）。在 MRSA 中鉴定出 3 种序列型（ST6、ST5 和 ST1），以 ST6 为主（n=7）。在甲氧西林敏感金黄色葡萄球菌中检测到 7 种不同的 ST 型[ST398、ST97、ST15、ST7、ST291、ST5043 和一个新的 ST（ST9219）]。所有 MRSA 分离株均携带 mecA 基因和 SCCmec-IVa 型。MRSA 对四环素[耐 3 株（tet(L) 和 tet(M)；1 株耐 tet(K)]、卡那霉素-妥布霉素[耐 3 株（ant(4')-Ia)]、阿米卡星-卡那霉素（耐 1 株/aph(3')-IIIa）和红霉素-克林霉素[耐 1 株/erm(C)]表现出耐药性。7 株金黄色葡萄球菌呈多重耐药。所有分离株均为 lukS/lukF-PV 和 tst-1 基因阴性，而 20 株分离株为 IEC 阳性。本研究表明，从宠物猫鼻腔样本中分离的金黄色葡萄球菌菌株存在遗传谱系多样性，包括多药耐药和产毒菌株。IEC 阳性金黄色葡萄球菌的存在提示可能存在人与动物的传播。
