School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan 410083, China; School of Resources and Environmental Engineering, Shanghai Polytechnic University, Shanghai 201209, China; School of Metallurgy and Environment, Central South University, Changsha, Hunan 410083, China.
School of Metallurgy and Environment, Central South University, Changsha, Hunan 410083, China.
Bioresour Technol. 2025 Jan;416:131794. doi: 10.1016/j.biortech.2024.131794. Epub 2024 Nov 9.
The high cost of harvesting microalgae limits their industrial application. Fungal-microalgal pellets can efficiently harvest microalgae and enhance heavy-metal adsorption. However, the molecular response mechanism of fungal-microalgal pellets under heavy-metal stress remains unclear. Fungal-microalgal pellets in a photobioreactor were used as a research object, and a 98 % harvesting efficiency could be achieved with adding exogenous carbon and nitrogen at pH 5.0-6.0 for 12 h of co-culture. Humic acid- and tryptophan-rich proteins in extracellular polymeric substances (EPS) participate in Cd(II) complexation. The Cd(II) response in fungal-microalgal pellets involves amino acids, glucose, lipids, energy metabolism, and antioxidant systems. The turning point was at 48 h. Proline, histidine, and glutamine synthesis and the adenosine-triphosphate (ATP) binding cassette (ABC) transport pathway play important roles in resistance to Cd(II) biotoxicity. This study provides a reference for the large-scale cultivation of fungal-microalgal symbiotic pellets and the practical application for industrial heavy-metal wastewater.
藻菌颗粒高效回收微藻并增强重金属吸附，但重金属胁迫下藻菌颗粒的分子响应机制尚不清楚。以光生物反应器中的藻菌颗粒为研究对象，在 pH 5.0-6.0 下添加外源碳氮共培养 12 h，可实现 98%的高效回收。胞外聚合物（EPS）中富含腐殖酸和色氨酸的蛋白质参与 Cd(II)配位。藻菌颗粒对 Cd(II)的响应涉及氨基酸、葡萄糖、脂类、能量代谢和抗氧化系统。转折点在 48 h。脯氨酸、组氨酸和谷氨酰胺的合成以及三磷酸腺苷（ATP）结合盒（ABC）转运途径在抵抗 Cd(II)生物毒性方面发挥重要作用。本研究为藻菌共生颗粒的大规模培养和工业重金属废水的实际应用提供了参考。
