Chestkov V V, Kugukova Iu V, Kovalev L I, Shishkin S S
Vopr Med Khim. 1986 Jan-Feb;32(1):123-7.
Electrophoretically homogenous phenylalanine hydroxylase was isolated from rat liver tissue using a number of methods involving affinity chromatography on phenyl-Sepharose, ion exchange chromatography on DEAE-Toyoperle and hydrophobic rechromatography on phenyl-Sepharose. Molecular mass of the enzyme was equal to 50,000 Da and Km = 4.4 X 10(-3) M with phenylalanine as a substrate. The enzymatic preparations were used for immunization of rabbits and the monospecific antiserum towards phenylalanine hydroxylase was produced.
使用多种方法从大鼠肝脏组织中分离出电泳纯的苯丙氨酸羟化酶，这些方法包括在苯基琼脂糖上进行亲和色谱、在DEAE- Toyoperle上进行离子交换色谱以及在苯基琼脂糖上进行疏水再色谱。该酶的分子量为50,000 Da，以苯丙氨酸为底物时Km = 4.4×10(-3) M。酶制剂用于免疫兔子，制备出了针对苯丙氨酸羟化酶的单特异性抗血清。
