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[测定环氧水解酶的荧光分光光度法]

[Spectrofluorimetric method of determining epoxide hydrolase].

作者信息

Kranauskas A E, Kravchenko L V, Tutel'ian V A

出版信息

Vopr Med Khim. 1986 Jan-Feb;32(1):37-9.

PMID:3953010
Abstract

A spectrofluorometric procedure is developed for estimation of epoxide hydrolase activity using styrol oxide as a substrate. The data were plotted, reflecting the dependence of the enzymatic reaction on time, pH value and the substrate concentration. Activity of epoxide hydrolase was studied in various tissues and subcellular fractions of rat liver tissue.

摘要

开发了一种荧光分光光度法,以氧化苯乙烯为底物来估计环氧水解酶的活性。绘制了数据,反映了酶促反应对时间、pH值和底物浓度的依赖性。在大鼠肝脏组织的各种组织和亚细胞组分中研究了环氧水解酶的活性。

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