Department of Genetics, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
Department of Genetics, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Vakif Gureba Cad, 34080, Sehremini, Istanbul, Turkey.
Mol Biol Rep. 2024 Nov 12;51(1):1144. doi: 10.1007/s11033-024-10091-w.
Obesity is associated with decreased ESR1 expression level in visceral adipose tissue. However, it is unclear exactly what mechanisms are responsible for this decline. The aim of this study was to investigate the impact of aberrant methylation of the ESR1 alternative promoters on decreased ESR1 expression and its connection to obesity.
Visceral adipose tissues and peripheral blood cells were obtained from 21 patients (non-obese and obese) undergoing inguinal hernia or gallbladder removal. Alternative promoter regions, C, E2 and F of the ESR1 gene, were analyzed by Methylation-Specific PCR (MSP) and mRNA levels were measured by quantitative real-time PCR (qPCR) in both visceral adipose tissue and peripheral blood cells. All statistical analyses were performed by SPSS (23.0).
The methylation percentage in the three promoter regions of ESR1 was not different in obese individuals compared to non-obese individuals. We observed that promoter C had the highest methylation frequency in obese patients, although it was not statistically significant. Additionally, we observed that the hypermethylation of ESR1's promoter C was significantly associated with lower mRNA expression level in obesity (p = 0.020).
This study suggests that methylation of ESR1 promoter C may be a factor in the development of obesity or a consequence of obesity. Further studies with advanced methods and larger study groups are needed to clarify this issue.
肥胖与内脏脂肪组织中 ESR1 表达水平降低有关。然而，具体是什么机制导致这种下降还不清楚。本研究旨在探讨 ESR1 替代启动子异常甲基化对 ESR1 表达降低的影响及其与肥胖的关系。
从 21 名（非肥胖和肥胖）接受腹股沟疝或胆囊切除术的患者中获取内脏脂肪组织和外周血细胞。通过甲基化特异性 PCR（MSP）分析 ESR1 基因的替代启动子区域 C、E2 和 F，通过定量实时 PCR（qPCR）测量内脏脂肪组织和外周血细胞中的 mRNA 水平。所有统计分析均由 SPSS（23.0）进行。
肥胖个体与非肥胖个体相比，ESR1 三个启动子区域的甲基化百分比没有差异。我们观察到肥胖患者中启动子 C 的甲基化频率最高，尽管这没有统计学意义。此外，我们观察到 ESR1 启动子 C 的高甲基化与肥胖患者中较低的 mRNA 表达水平显著相关（p=0.020）。
本研究表明，ESR1 启动子 C 的甲基化可能是肥胖发生的一个因素，或者是肥胖的结果。需要使用更先进的方法和更大的研究组进一步研究，以阐明这个问题。
