Department of Clinical Laboratory, Gongli Hospital of Shanghai Pudong New Area, Shanghai, China.
Dongming Community Health Service Center, Pudong New Area, Shanghai, China.
PLoS One. 2024 Nov 12;19(11):e0312773. doi: 10.1371/journal.pone.0312773. eCollection 2024.
Glutamine cyclase, an enzyme involved in posttranslational modifications, is encoded by the glutaminyl-peptide cyclotransferase (QPCT) gene. Gene microarray analysis revealed that the QPCT gene was highly expressed in HepG2.2.15 cells compared with that in HepG2 cells. The serum expression level of the QPCT gene was detected by ELISA and was significantly greater in HBV-infected patients than in healthy controls. The mRNA and protein expression levels of the QPCT gene were markedly greater in the HBV-expressing cell lines (HepG2.2.15, and HepG2 and Huh7 cells transfected with the pBlu-HBV plasmid) than in the HepG2 and Huh7 cells. The levels of HBV pgRNA and HBV-DNA copy number, as well as the levels of HBeAg and HBsAg, also increased in the HepG2 and Huh7 cell lines cotransfected with the QPCT gene expression plasmid and the HBV 1.3-fold plasmid. Our study indicated that HBV can promote the expression of the QPCT gene, which in turn promotes the expression and replication of HBV.
谷氨酰胺环化酶是一种参与翻译后修饰的酶，由谷氨酰胺肽环转移酶（QPCT）基因编码。基因微阵列分析显示，QPCT 基因在 HepG2.2.15 细胞中的表达水平明显高于 HepG2 细胞。通过 ELISA 检测血清中 QPCT 基因的表达水平，发现乙型肝炎病毒（HBV）感染患者明显高于健康对照组。HBV 表达细胞系（HepG2.2.15 细胞和转染 pBlu-HBV 质粒的 HepG2 和 Huh7 细胞）中 QPCT 基因的 mRNA 和蛋白表达水平明显高于 HepG2 和 Huh7 细胞。在共转染 QPCT 基因表达质粒和 HBV 1.3 倍质粒的 HepG2 和 Huh7 细胞系中，HBV pgRNA 和 HBV-DNA 拷贝数以及 HBeAg 和 HBsAg 的水平也升高。我们的研究表明，HBV 可以促进 QPCT 基因的表达，进而促进 HBV 的表达和复制。
