Chhetri Srijana B, Nance Daniel, Loya Mwajabu, Cornaby Caleb, Markmann Alena J, Schmitz John L, Lin Jessica T, Ngasala Billy
Institute of Global Health and Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, North Carolina.
Department of Parasitology and Medical Entomology, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania.
Am J Trop Med Hyg. 2024 Nov 12;112(1):177-181. doi: 10.4269/ajtmh.23-0784. Print 2025 Jan 8.
In the first 6 months of the coronavirus disease 2019 pandemic, limited testing clouded understanding of the extent of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission in Africa. In particular, Tanzania halted all testing and reporting of SARS-CoV-2 cases after May 2020, not resuming until June 2021. In July-August 2020, we performed a seroprevalence survey in rural Bagamoyo district, 40 km outside Dar es Salaam. Among 347 asymptomatic children and adults, 64/347 (18.0%) demonstrated seroreactivity to SARS-CoV-2 spike receptor binding domain by ELISA. Given significant antibody cross-reactivity in malaria-endemic regions, seropositivity was additionally confirmed via a multitarget Luminex immunoassay. Thirty-seven, or 58% of initially seroreactive persons, were Luminex positive, leading to an estimated SARS-CoV-2 seroprevalence of 10.7% (37/347, 95% CI 7.6-14.4%). Working in health care appeared to be associated with seropositivity. Reporting of viral symptoms or health care-seeking behavior in the previous 3 months was not more frequent in seropositive individuals.
在2019年冠状病毒病大流行的头6个月里，检测受限使得人们对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）在非洲的传播程度了解不足。特别是，坦桑尼亚在2020年5月之后停止了所有SARS-CoV-2病例的检测和报告，直到2021年6月才恢复。2020年7月至8月，我们在距离达累斯萨拉姆40公里的巴加莫约农村地区进行了一项血清流行率调查。在347名无症状儿童和成人中，通过酶联免疫吸附测定（ELISA），64/347（18.0%）的人对SARS-CoV-2刺突受体结合域呈血清反应性。鉴于疟疾流行地区存在显著的抗体交叉反应性，还通过多靶点Luminex免疫测定法进一步确认了血清阳性。37名最初血清反应阳性者中，有37名（58%）Luminex检测呈阳性，导致估计的SARS-CoV-2血清流行率为10.7%（37/347，95%置信区间7.6 - 14.4%）。从事医疗保健工作似乎与血清阳性有关。血清阳性个体在过去3个月中报告病毒症状或寻求医疗行为的频率并不更高。
