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暴露于阳光与未暴露皮肤之间细胞亚群的差异：来自单个病例的初步单细胞测序及生物学分析

Differences in cell subsets between sun-exposed and unexposed skin: preliminary single-cell sequencing and biological analysis from a single case.

作者信息

Zhou Feng, Sun Yu, Chen Xinling, Hou Wenyi, Shen Jing, Lai Wei, Han Kai, Zheng Yue

机构信息

Department of Dermato-venereology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

出版信息

Front Med (Lausanne). 2024 Oct 29;11:1453940. doi: 10.3389/fmed.2024.1453940. eCollection 2024.

DOI:10.3389/fmed.2024.1453940
PMID:39540047
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11558528/
Abstract

INTRODUCTION

The composition and subsets of skin cells continuously change in a dynamic manner. However, the specific microcosmic alterations of human photoaged skin, independent of chronologic aging, remain unclear and have been infrequently analyzed. This study aimed to evaluate the biological processes and mechanisms underlying cell-subgroup alterations in skin photoaging.

METHODS

We utilized single-cell sequencing and biological analysis from a single case to investigate the effects of photoaging. Skin punch biopsies were taken from sun-exposed forearm skin and unexposed buttock skin from the same individual for comparative analysis.

RESULTS

Our analysis identified 25 cell clusters and 12 skin cell types, revealing significant changes in unique gene expressions between the sun-exposed and unexposed skin samples. A comparison of cell numbers within each cluster revealed 9 dominant cell clusters in sun-exposed skin and 16 dominant cell clusters in unexposed skin. Enrichment analysis indicated that PD-L1 expression and the PD-1 checkpoint pathway were more prominent in sun-exposed skin, while MAPK, TNF-alpha, TGF-beta, and apoptosis pathways were more enriched in hair follicle cells of sun-exposed skin.

DISCUSSION

This study reveals changes in cell components in photoaged skin from a single case and provides novel insights into cellular subpopulations and pathology during repeated UVA-induced skin damage. These findings enhance our understanding of the complex interplay between different cells in photoaged skin and offer potential targets for preventing human skin photoaging and UV-induced skin cancers.

摘要

引言

皮肤细胞的组成和亚群以动态方式持续变化。然而，与自然衰老无关的人类光老化皮肤的具体微观变化仍不清楚，且很少被分析。本研究旨在评估皮肤光老化中细胞亚群改变的生物学过程和机制。

方法

我们利用单细胞测序和单病例生物学分析来研究光老化的影响。从同一个体暴露于阳光的前臂皮肤和未暴露的臀部皮肤取皮肤打孔活检组织进行对比分析。

结果

我们的分析鉴定出25个细胞簇和12种皮肤细胞类型，揭示了暴露于阳光和未暴露皮肤样本之间独特基因表达的显著变化。对每个簇内细胞数量的比较显示，暴露于阳光的皮肤中有9个主要细胞簇，未暴露皮肤中有16个主要细胞簇。富集分析表明，PD-L1表达和PD-1检查点通路在暴露于阳光的皮肤中更突出，而MAPK、TNF-α、TGF-β和凋亡通路在暴露于阳光的皮肤的毛囊细胞中更富集。

讨论

本研究揭示了单病例光老化皮肤中细胞成分的变化，并为反复UVA诱导的皮肤损伤期间的细胞亚群和病理学提供了新见解。这些发现增进了我们对光老化皮肤中不同细胞之间复杂相互作用的理解，并为预防人类皮肤光老化和紫外线诱导的皮肤癌提供了潜在靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8632/11558528/4dbe5a30103d/fmed-11-1453940-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8632/11558528/a0f8b88b2470/fmed-11-1453940-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8632/11558528/b79d1f8c5a46/fmed-11-1453940-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8632/11558528/7215d6d0db4b/fmed-11-1453940-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8632/11558528/191e1e06741a/fmed-11-1453940-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8632/11558528/6f1b739152af/fmed-11-1453940-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8632/11558528/4dbe5a30103d/fmed-11-1453940-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8632/11558528/a0f8b88b2470/fmed-11-1453940-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8632/11558528/b79d1f8c5a46/fmed-11-1453940-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8632/11558528/7215d6d0db4b/fmed-11-1453940-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8632/11558528/191e1e06741a/fmed-11-1453940-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8632/11558528/6f1b739152af/fmed-11-1453940-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8632/11558528/4dbe5a30103d/fmed-11-1453940-g006.jpg

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