使用mRNA重编程方法从健康人类血管生成血液内皮细胞（BOECs）中生成CTRL EiPS J9 mR6F-8诱导多能干细胞系。
Generation of the CTRL EiPS J9 mR6F-8 iPSC line derived from healthy human outgrowth blood endothelial cells (BOECs) using mRNA reprogramming methodology.
作者信息
Selvitella Silvia, Franco Marta Pérez, Aran Begoña, Veiga Anna, Kuebler Bernd
机构信息
Barcelona Stem Cell Bank, Spain; Regenerative Medicine Program, Institut d'Investigació Biomèdica, IDIBELL, Spain; P-CMR[C]-Program of Translation of Regenerative Medicine in Catalonia, Spain.
Barcelona Stem Cell Bank, Spain; Regenerative Medicine Program, Institut d'Investigació Biomèdica, IDIBELL, Spain; P-CMR[C]-Program of Translation of Regenerative Medicine in Catalonia, Spain.
出版信息
Stem Cell Res. 2024 Dec;81:103606. doi: 10.1016/j.scr.2024.103606. Epub 2024 Nov 9.
mRNA reprogramming is a technology for generating iPSCs with high efficiency and safety. However, it is not suitable for reprogramming non-adherent cells, the primary cell type in blood. An alternative is to obtain adherent cells from blood for this technology. To validate this approach, we generated a human iPSC line from blood outgrowth endothelial cells (BOECs) using mRNA-based reprogramming. The resulting line, CTRL EiPS J9 mR6F-8, meets iPSC criteria, including an undifferentiated state, pluripotency, genome integrity. This line is available upon request.
信使核糖核酸重编程是一种高效且安全地生成诱导多能干细胞的技术。然而，它不适用于对非贴壁细胞（血液中的主要细胞类型）进行重编程。一种替代方法是从血液中获取贴壁细胞用于该技术。为验证这种方法，我们使用基于信使核糖核酸的重编程技术从血液来源的内皮细胞（BOECs）中生成了一条人诱导多能干细胞系。所得的细胞系CTRL EiPS J9 mR6F-8符合诱导多能干细胞的标准，包括未分化状态、多能性和基因组完整性。可应要求提供该细胞系。
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