Department of Life Sciences - Food and Nutrition Science, Chalmers University of Technology, Gothenburg, Sweden.
Department of Marine Sciences - Tjärnö Marine Laboratory, University of Gothenburg, Strömstad, Sweden.
Food Chem. 2025 Feb 1;464(Pt 3):141839. doi: 10.1016/j.foodchem.2024.141839. Epub 2024 Oct 30.
Alternative protein sources such as seaweed can help relieve the pressure on land-based protein supply. This proof-of-concept study developed an extraction method to recover soluble and lipophilic proteins from the seaweed Ulva fenestrata. The method consisted of processing U. fenestrata with 0.1-0.5 % aqueous Triton X-114 solution and reprocessing the pellet with an alkaline aqueous solution. Then, the solubilized proteins were precipitated via acidification. The new method extracted 3.4-times more protein, measured as total amino acids, compared to the control with two alkaline aqueous extraction cycles. Triton disrupted the chloroplasts and likely solubilized lipophilic membrane proteins as supported by microstructure and polypeptide pattern analysis. Triton-derived protein extracts contained lipids inside the precipitates/aggregates and were richer in fatty acids typical of photosynthetic membranes. The higher extraction yields are proposed to result from membrane charge neutralization upon acidification, triggering interactions between the membrane lipids and their subsequent precipitation with the lipophilic membrane protein.
替代蛋白质来源，如海藻，可以帮助缓解对陆地蛋白质供应的压力。本概念验证研究开发了一种从海藻石莼中提取可溶和脂溶性蛋白质的方法。该方法包括用 0.1-0.5%的 Triton X-114 水溶液处理 U. fenestrata，然后用碱性水溶液对沉淀进行再处理。然后，通过酸化将溶解的蛋白质沉淀。与使用两种碱性水溶液提取循环的对照组相比，新方法提取的总氨基酸蛋白增加了 3.4 倍。Triton 通过破坏叶绿体并可能溶解脂溶性膜蛋白来支持微观结构和多肽模式分析。Triton 衍生的蛋白质提取物在沉淀物/聚集体中含有内部的脂质，并且富含典型的光合膜脂肪酸。提出更高的提取产率是由于酸化时膜电荷中和，触发膜脂及其随后与脂溶性膜蛋白沉淀之间的相互作用。
