Wang Man, Jiang Muqi, Wang Qi, Sun Yasheng, Nie Zhixiang, Palin William M, Zhang Zhen
Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan 430022, China.
Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Dental and Biomaterials Sciences, School of Dentistry, Institute of Clinical Sciences, College of Medical and Dental Sciences, University of Birmingham, UK.
Biomater Adv. 2025 Feb;167:214110. doi: 10.1016/j.bioadv.2024.214110. Epub 2024 Nov 8.
This study aims to explore the efficacy of Electrophoretic Deposition (EPD) for collagen type I coating on titanium implants and its subsequent mineralization to improve osseointegration and bone regeneration.
Titanium disks were prepared with a sandblasted, large grit and acid-etched (SLA) surface. EPD was employed to deposit collagen type I onto the titanium surfaces, followed by two modes of mineralization: extra-fibril mineralization (EFM) and inter-fibril mineralization (IFM). Then comprehensive in vitro studies were conducted including surface properties, cell proliferation, osteogenic differentiation, and inflammatory responses.
EPD successfully deposited a uniform collagen layer on titanium surfaces. EFM resulted in deposition of larger, irregularly shaped crystals, while IFM produced controlled, helical fibril mineralization. IFM-treated surfaces exhibited enhanced cell viability, proliferation, and osteogenic differentiation. Both EFM and IFM surfaces triggered higher macrophage activation than SLA surfaces. While EFM primarily induced a stronger M1 pro-inflammatory response, IFM exhibited a more balanced macrophage polarization with upregulated M2 markers at later stages.
EPD, particularly when integrated with IFM, significantly enhances the bioactivity and osteogenic potential of collagen-coated titanium implants. This method surpasses traditional SLA surfaces by stabilizing the collagen layer and creating a biomimetic environment conducive to bone regeneration and healing through a balanced inflammatory response, offering a promising strategy to improve titanium implant performance.
本研究旨在探讨电泳沉积（EPD）法用于在钛种植体上涂覆Ⅰ型胶原蛋白及其随后矿化以改善骨整合和骨再生的效果。
制备具有喷砂、大颗粒和酸蚀（SLA）表面的钛盘。采用EPD法将Ⅰ型胶原蛋白沉积到钛表面，随后进行两种矿化模式：原纤维外矿化（EFM）和原纤维间矿化（IFM）。然后进行了包括表面性质、细胞增殖、成骨分化和炎症反应在内的全面体外研究。
EPD成功地在钛表面沉积了一层均匀的胶原蛋白层。EFM导致沉积更大、形状不规则的晶体，而IFM产生可控的螺旋状纤维矿化。经IFM处理的表面表现出增强的细胞活力、增殖和成骨分化。EFM和IFM处理的表面均比SLA表面引发更高的巨噬细胞活化。虽然EFM主要诱导更强的M1促炎反应，但IFM表现出更平衡的巨噬细胞极化，后期M2标志物上调。
EPD，特别是与IFM结合时，显著增强了胶原蛋白涂层钛种植体的生物活性和成骨潜力。该方法通过稳定胶原蛋白层并通过平衡的炎症反应创造有利于骨再生和愈合的仿生环境，超越了传统的SLA表面，为改善钛种植体性能提供了一种有前景的策略。
