Qiao Pengfang, Zhao Maoqiu, Zhao Jialiang, Wen Jiayin, Zhao Cuizhu, Zhang Meng
College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.
Gene. 2025 Feb 5;936:149085. doi: 10.1016/j.gene.2024.149085. Epub 2024 Nov 13.
The membrane-bound O-acyltransferase (MBOAT) gene family comprises enzymes responsible for transferring acyl groups to various lipid molecules. Some members of the MBOAT gene family and their functions have been extensively studied in the model plant Arabidopsis. However, research on the MBOAT gene family in camelina is still limited. In this study, 54 MBOATs were identified on 17 chromosomes and one unidentified scaffold in camelina, including seven newly identified genes. A total of 149 MBOATs were identified in 10 other species. Six subgroups of these MBOATs with different conservation were classified by phylogenetic analysis, showing diversification between monocots and dicots. Detailed analysis of the motif composition, evolutionary relationships, and gene structures of CsaMBOATs are presented. The results of the syntenic analysis suggest that the evolution of CsaMBOAT gene family is primarily driven by segmental and tandem duplications, and that there is a stronger collinearity within dicots. In addition, analysis of CsaMBOAT gene promoter cis-elements reveals a possible transcriptional regulation and tissue-specific expression, highlighting potential role in plant stress responses and hormone signaling. Furthermore, both the transcriptome and RT-qPCR data revealed the changes in the expression levels of DGAT1 during salt stress treatment. Subsequent analyses indicated that DGAT1 influenced the ratio of fatty acid fractions in the plants. Importantly, a large number of transcription factors involved in the regulation of CsaMBOAT gene expression were identified by WGCNA analysis, and the transcriptional data confirmed that the NAC032 and CAMMTA6 genes play a role upstream of DGAT1. This study not only identified the members of the MBOAT in camelina, but also provided insights and clues into its regulatory mechanisms.
膜结合O-酰基转移酶（MBOAT）基因家族包含负责将酰基转移到各种脂质分子的酶。MBOAT基因家族的一些成员及其功能已在模式植物拟南芥中得到广泛研究。然而，关于亚麻荠中MBOAT基因家族的研究仍然有限。在本研究中，在亚麻荠的17条染色体和一个未鉴定的支架上鉴定出54个MBOAT，其中包括7个新鉴定的基因。在其他10个物种中总共鉴定出149个MBOAT。通过系统发育分析将这些MBOAT分为六个具有不同保守性的亚组，显示出单子叶植物和双子叶植物之间的多样性。本文对CsaMBOAT的基序组成、进化关系和基因结构进行了详细分析。共线性分析结果表明，CsaMBOAT基因家族的进化主要由片段重复和串联重复驱动，并且双子叶植物内部具有更强的共线性。此外，对CsaMBOAT基因启动子顺式元件的分析揭示了可能的转录调控和组织特异性表达，突出了其在植物应激反应和激素信号传导中的潜在作用。此外，转录组和RT-qPCR数据均揭示了盐胁迫处理期间DGAT1表达水平的变化。随后的分析表明，DGAT1影响植物中脂肪酸组分的比例。重要的是，通过WGCNA分析鉴定了大量参与CsaMBOAT基因表达调控的转录因子，转录数据证实NAC032和CAMMTA6基因在DGAT1上游发挥作用。本研究不仅鉴定了亚麻荠中MBOAT的成员，还为其调控机制提供了见解和线索。
