Zare-Gachi Maryam, Sadeghi Amin, Choshali Mahmoud Alipour, Ghadimi Tayyeb, Forghani Siamak Farokh, Pezeshki-Modaress Mohamad, Daemi Hamed
Zharfandishan Fanavar Zistbaspar (ZFZ) Chemical Company, Tehran, Iran.
Soft Tissue Engineering Research Center, Tissue Engineering and Regenerative Medicine Institute, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Int J Biol Macromol. 2024 Dec;283(Pt 2):137557. doi: 10.1016/j.ijbiomac.2024.137557. Epub 2024 Nov 13.
Diabetic foot ulcer (DFU) is a chronic and non-healing wound in all age categories with a high prevalence and mortality in the world. An ideal wound dressing for DFU should possess the ability of adsorbing high contents of exudate and actively promote wound healing. Here, we introduced the calcium alginate sulfate as a new biomaterial appropriate for use in wound dressing to promote the healing of full-thickness ulcers in a diabetic mouse model. In this regard, alginate sulfate (Alg-S) solutions with different degrees of substitution (DS) of 0.2, 0.5, and 0.9 were synthesized, freeze-dried, crosslinked by calcium cations, purified by washing and refreeze-dried. Primary analyses including swelling ratio, porosity content and mechanical properties revealed that all Alg-S scaffolds possess necessities for use as a wound dressing. After confirming the cytocompatibility of both alginate and alginate sulfate-based scaffolds by MTT assay, they were used as wound dressing for healing of full-thickness ulcers in diabetic mice. The results of wound healing process confirmed that calcium alginate sulfate scaffolds can heal the wounds faster than both alginate-treated and non-treated wounds. Furthermore, the histological analyses of healed tissues reveled normal regeneration of the skin tissue layers and collagen deposition similar to the healthy tissue.
糖尿病足溃疡（DFU）是一种在所有年龄段都存在的慢性难愈合伤口，在全球范围内具有较高的患病率和死亡率。一种理想的DFU伤口敷料应具备吸附大量渗出液的能力，并能积极促进伤口愈合。在此，我们引入硫酸海藻酸钙作为一种适用于伤口敷料的新型生物材料，以促进糖尿病小鼠模型中全层溃疡的愈合。在这方面，合成了不同取代度（DS）分别为0.2、0.5和0.9的硫酸海藻酸盐（Alg-S）溶液，进行冷冻干燥，通过钙阳离子交联，经洗涤纯化后再冷冻干燥。包括膨胀率、孔隙率和力学性能在内的初步分析表明，所有Alg-S支架都具备用作伤口敷料的必要条件。通过MTT法确认海藻酸盐和硫酸海藻酸盐基支架的细胞相容性后，将它们用作糖尿病小鼠全层溃疡愈合的伤口敷料。伤口愈合过程的结果证实，硫酸海藻酸钙支架比海藻酸盐处理组和未处理组的伤口愈合得更快。此外，愈合组织的组织学分析显示，皮肤组织层正常再生，胶原沉积与健康组织相似。
