Department of Pharmaceutical, Chemical & Environmental Sciences, Faculty of Engineering and Science, University of Greenwich, Medway, Central Ave. Chatham Maritime, Kent, ME4 4TB, UK.
Drug Deliv Transl Res. 2018 Dec;8(6):1751-1768. doi: 10.1007/s13346-017-0445-9.
Calcium alginate (CA) wafer dressings were prepared by lyophilization of hydrogels to deliver ciprofloxacin (CIP) directly to the wound site of infected diabetic foot ulcers (DFUs). The dressings were physically characterized by scanning electron microscopy (SEM), texture analysis (for mechanical and in vitro adhesion properties), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). Further, functional properties essential for wound healing, i.e., porosity, in vitro swelling index, water absorption (Aw), equilibrium water content (EWC), water vapor transmission rate (WVTR), evaporative water loss (EWL), moisture content, in vitro drug release and kinetics, antimicrobial activity, and cell viability (MTT assay) were investigated. The wafers were soft, of uniform texture and thickness, and pliable in nature. Wafers showed ideal wound dressing characteristics in terms of fluid handling properties due to high porosity (SEM). XRD confirmed crystalline nature of the dressings and FTIR showed hydrogen bond formation between CA and CIP. The dressings showed initial fast release followed by sustained drug release which can inhibit and prevent re-infection caused by both Gram-positive and Gram-negative bacteria. The dressings also showed biocompatibility (> 85% cell viability over 72 h) with human adult keratinocytes. Therefore, it will be a potential medicated dressing for patients with DFUs infected with drug-resistant bacteria.
海藻酸钙（CA）水凝胶经冷冻干燥制备成载药敷料，可将环丙沙星（CIP）直接递送至感染的糖尿病足溃疡（DFU）创面。采用扫描电子显微镜（SEM）、质构分析（用于机械和体外粘附性能）、X 射线衍射（XRD）和傅里叶变换红外光谱（FTIR）对敷料进行物理特性表征。此外，还研究了对伤口愈合至关重要的功能特性，即孔隙率、体外溶胀指数、吸水率（Aw）、平衡含水量（EWC）、水蒸气透过率（WVTR）、蒸发失水（EWL）、水分含量、体外药物释放和动力学、抗菌活性和细胞活力（MTT 测定）。水凝胶敷料柔软、质地和厚度均匀、柔韧性好。水凝胶敷料由于具有较高的孔隙率（SEM），在处理流体方面表现出理想的伤口敷料特性。XRD 证实了敷料的结晶性质，FTIR 表明 CA 和 CIP 之间形成了氢键。该敷料表现出初始快速释放，随后是持续的药物释放，可以抑制和预防革兰氏阳性和革兰氏阴性细菌引起的再次感染。该敷料对人成角质细胞也具有生物相容性（72 h 后细胞活力>85%）。因此，它将成为一种有潜力的治疗糖尿病足溃疡感染耐药菌患者的药物敷料。
