利用蛋白质组学生物信息学从雨叶花花粉中鉴定潜在过敏原。

Identifying putative allergens from Cenostigma pluviosum pollen using proteomic bioinformatics.

机构信息

Graduate Program in Research and Development (Medical Biotechnology, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, SP, 18618-687, Brazil.

Department of Pediatrics, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, SP, 18618-687, Brazil.

出版信息

Mol Biol Rep. 2024 Nov 14;51(1):1155. doi: 10.1007/s11033-024-10079-6.

DOI:10.1007/s11033-024-10079-6

Abstract

BACKGROUND

Routinely, signs and symptoms of pollinosis worsen during the flowering period of the Cenostigma pluviosum var. peltophoroides (Sibipiruna) trees. This study aimed to determine whether Sibipiruna pollen contains allergenic proteins.

METHODS AND RESULTS

The pollen from mature Sibipiruna anthers was morphologically characterized using optical, scanning electron and emission microscopy. Additionally, biochemical characterization was conducted through Shotgun Label-Free Proteomic Analysis. Three hundred and forty-nine proteins were identified using the UNIPROT database and thirty-six were confirmed as homologous allergenic proteins in the Allergome database.

CONCLUSIONS

At the first time, Sibipiruna pollen allergenic proteins have been described and this study provides a deeper understanding of the allergenic proteins present in this pollen. These preliminary findings can be useful to assist in the development of targeted extracts for the study of sensitization and potential immunotherapy in the future.

摘要

背景

花粉症的症状和体征通常会在 Cenostigma pluviosum var. peltophoroides（西比皮鲁纳）树开花期间加重。本研究旨在确定西比皮鲁纳花粉是否含有过敏原蛋白。

方法和结果

使用光学显微镜、扫描电子显微镜和发射显微镜对成熟西比皮鲁纳花粉花药的花粉进行了形态学特征描述。此外，还通过 Shotgun Label-Free Proteomic Analysis 进行了生化特征描述。使用 UNIPROT 数据库鉴定了 349 种蛋白质，在 Allergome 数据库中确认了 36 种同源过敏原蛋白。