Department of Plant Protection, Wroclaw University of Environmental and Life Sciences, Grunwaldzki Sq. 24a, Wroclaw, 53-363, Poland.
Department of Ecology and Environment, Abbes Laghrour University Khenchela, Khenchela, 40000, Algeria.
Sci Rep. 2024 Nov 14;14(1):27998. doi: 10.1038/s41598-024-79766-5.
Collembola play a key role in soil ecosystems by decomposing organic matter. Most of them inhabit the upper layers of the soil and are susceptible to contamination present in the pore water. These arthropods serve as model organisms in ecotoxicology for short and long-term exposure. This study aimed to assess the toxicity of three heavy metals (lead [Pb], cadmium [Cd], and copper [Cu]) using the springtail species Folsomia candida as the test organism, with mortality and growth inhibition as the measure of toxicity. We hypothesised that increasing metal concentrations in the soil would correspond to a growth reduction of Collembola. Each heavy metal was tested at a minimum of eight increasing concentrations in six replications. Twenty 10-12-day-old individuals were introduced into each test container filled with contaminated or control soil and incubated for 14 days. The test endpoints included growth inhibition determined by comparing F. candida growth rates in contaminated soil with those in control soil, as well as mortality rates. The EC values (mg/kg) for heavy metals were as follows: Cd = 66.89, Cu = 791.01, Pb = 10075.48. Our findings suggest that growth inhibition is a reliable indicator of Collembola toxicity to heavy metals.
跳虫在土壤生态系统中通过分解有机物起着关键作用。它们中的大多数栖息在土壤的上层，容易受到孔隙水中存在的污染物质的影响。这些节肢动物作为生态毒理学中的模式生物，可用于短期和长期暴露的研究。本研究旨在使用跳虫物种 Folsomia candida 评估三种重金属（铅[Pb]、镉[Cd]和铜[Cu]）的毒性，死亡率和生长抑制作为毒性的衡量标准。我们假设土壤中重金属浓度的增加将对应于 Collembola 的生长减少。每种重金属在 6 个重复的至少 8 个递增浓度下进行测试。将 20 只 10-12 天大的个体引入每个装有污染或对照土壤的测试容器中，并在 14 天内进行孵育。测试终点包括通过比较污染土壤和对照土壤中 F. candida 的生长率来确定生长抑制，以及死亡率。重金属的 EC 值（mg/kg）如下：Cd=66.89，Cu=791.01，Pb=10075.48。我们的研究结果表明，生长抑制是重金属对 Collembola 毒性的可靠指标。
