Department of Physics, Faculty of Converging Sciences and Technologies, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Seed and Plant Certification and Registration Research Institute (SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
BMC Biotechnol. 2024 Nov 14;24(1):93. doi: 10.1186/s12896-024-00921-x.
Cold atmospheric pressure plasma (CAP) has garnered significant attention in recent years for its potential applications in biomedical, environmental, and agricultural fields. Cold plasma treatment exhibits a variety of effects in agricultural applications, including impacts on seed germination and seedling growth; however, further research is required. Soybean serves as a fundamental source of nutrients for both animals and humans. Soybean seeds possess impermeable and thick testae, which results in prolonged germination times and suboptimal germination rates. The soybeans exhibit low uniformity. As a result, poor crop establishment and yield reduction are inevitable outcomes. Therefore, the purpose of this study was to examine the effects of Iranian soybean cultivars, such as Sari, Saba, Arian, Katoul, and Williams, on seedling growth properties, seed germination, and antioxidant enzyme activity, using argon at time intervals of 30, 60, 180, 300, and 420 s. Cold plasma treatment significantly enhanced germination potential from 1.18 to 66.97%, germination index from 0.50 to 60.09%, germination rate from 1.78 to 32.17%, seedling length from 2.70 cm to 78.13 cm, root length from 2.87 cm to 56.13 cm, and seedling dry weight from 1.80 g to 36.63 g. Additionally, CAT activity increased from 0.88- to 4.40-fold, SOD activity from 0.86- to 5.89-fold, and APX activities from 0.40- to 4.01-fold compared to the control treatment. The findings indicated that the samples exhibited optimal results at treatment durations of 60 and 180 s. The influence of plasma on the antioxidant responses of seedlings, seed germination, and growth characteristics was contingent upon the duration of treatment. Cold plasma, when applied for an appropriate duration, may enhance soybean seedling growth characteristics and seed germination.
冷大气压等离子体（CAP）在近年来因其在生物医学、环境和农业领域的潜在应用而受到广泛关注。冷等离子体处理在农业应用中表现出多种效果，包括对种子萌发和幼苗生长的影响；然而，需要进一步的研究。大豆是动物和人类基本的营养来源。大豆种子具有不透水和厚种皮，导致萌发时间延长和萌发率不理想。大豆表现出低均匀性。因此，不可避免地会出现作物生长不良和产量降低的情况。因此，本研究旨在探讨伊朗大豆品种，如 Sari、Saba、Arian、Katoul 和 Williams，对幼苗生长特性、种子萌发和抗氧化酶活性的影响，使用氩气处理时间间隔为 30、60、180、300 和 420 秒。冷等离子体处理显著提高了发芽势从 1.18%到 66.97%，发芽指数从 0.50%到 60.09%，发芽率从 1.78%到 32.17%，幼苗长度从 2.70 厘米到 78.13 厘米，根长从 2.87 厘米到 56.13 厘米，幼苗干重从 1.80 克到 36.63 克。此外，CAT 活性增加了 0.88 至 4.40 倍，SOD 活性增加了 0.86 至 5.89 倍，APX 活性增加了 0.40 至 4.01 倍，与对照处理相比。结果表明，在处理时间为 60 和 180 秒时，样品表现出最佳结果。等离子体对幼苗抗氧化反应、种子萌发和生长特性的影响取决于处理时间的长短。当冷等离子体应用于适当的时间时，可能会增强大豆幼苗的生长特性和种子萌发。
