瑞香素通过体外抑制 cGAS/STING 通路对 H3N2 流感病毒的潜在抗病毒活性。

Potential antiviral activity of rhamnocitrin against influenza virus H3N2 by inhibiting cGAS/STING pathway in vitro.

机构信息

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510180, China.

Institute of Integration of Traditional and Western Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

出版信息

Sci Rep. 2024 Nov 16;14(1):28287. doi: 10.1038/s41598-024-79788-z.

Influenza remains a serious issue for public health and it's urgent to discover more effected drugs against influenza virus. Rhamnocitrin, as a flavonoid, its effect on influenza virus infection remains poorly explored. In this study, rhamnocitrin showed antiviral effect and anti-apoptosis on influenza virus A/Aichi/2/1968 (H3N2) in MDCK cells and A549 cells. In addition, molecular docking revealed that rhamnocitrin have good binding activity with the target proteins cGAS and STING, molecular dynamic simulation and surface plasmon resonance showed that rhamnocitrin could form a stable complex with the above proteins. Moreover, the qPCR and western blot assays further verified that rhamnocitrin could reduce type I IFN and proinflammatory cytokines production by inhibiting the cGAS/STING pathway. Taken together, the results suggest that rhamnocitrin could be a potential anti-viral agent against influenza.

流感仍然是一个严重的公共卫生问题，急需发现更有效的抗流感病毒药物。鼠李柠檬素作为一种类黄酮，其对流感病毒感染的作用仍未得到充分探索。在这项研究中，鼠李柠檬素在 MDCK 细胞和 A549 细胞中对甲型流感病毒 A/Aichi/2/1968（H3N2）显示出抗病毒作用和抗凋亡作用。此外，分子对接表明鼠李柠檬素有与靶蛋白 cGAS 和 STING 良好的结合活性，分子动力学模拟和表面等离子体共振表明鼠李柠檬素可以与上述蛋白形成稳定的复合物。此外，qPCR 和 western blot 检测进一步证实，鼠李柠檬素通过抑制 cGAS/STING 通路，减少 I 型 IFN 和促炎细胞因子的产生。综上所述，结果表明鼠李柠檬素可能是一种有潜力的抗流感病毒药物。