来自美国北卡罗来纳州马塔马斯基特湖一次水华中产毒蓝藻的基因组序列。

Genome sequences of toxigenic cyanobacteria from a bloom in Lake Mattamuskeet, North Carolina (United States).

作者信息

Moretto Jéssica A, Berthold David E, Lefler Forrest W, Mazzei Viviana, Loftin Keith A, Laughinghouse H Dail

机构信息

Agronomy Department, Fort Lauderdale Research and Education Center, University of Florida, Institute of Food and Agricultural Sciences, Davie, Florida, USA.

U.S. Geological Survey, Orlando, Florida, USA.

出版信息

J Phycol. 2024 Dec;60(6):1349-1355. doi: 10.1111/jpy.13523. Epub 2024 Nov 17.

DOI:10.1111/jpy.13523

PMID:39550760

Abstract

Lake Mattamuskeet, the largest lake in North Carolina, USA, has undergone decades-long eutrophication causing reduced water quality and promoting cyanobacterial blooms that may produce toxins. It is therefore necessary to evaluate the cyanobacterial diversity of the lake and their toxigenic potential. We present draft genomes of Microcystis, Pelatocladus, Raphidiopsis, and Umezakia strains isolated from Lake Mattamuskeet. The whole-genome shotgun projects for Umezakia ovalisporum BLCC-F208, Microcystis sp. BLCC-F209, Microcystis sp. BLCC-F210, Pelatocladus sp. BLCC-F211, U. ovalisporum BLCC-F215, and Raphidiopsis BLCC-F218 have been deposited in GenBank under accession numbers JBHFLK000000000, JBHFLL000000000, CP169647, JBHFLM000000000, JBHFLN000000000, and JBHFLO000000000, respectively. Based on the genomic analysis, several biosynthetic gene clusters (BCGs) with varying degrees of similarity to known toxic and bioactive compound gene clusters were identified across the different cyanobacterial strains.

摘要

美国北卡罗来纳州最大的湖泊马塔马斯基特湖经历了数十年的富营养化，导致水质下降，并促使蓝藻大量繁殖，可能产生毒素。因此，有必要评估该湖的蓝藻多样性及其产毒潜力。我们展示了从马塔马斯基特湖分离出的微囊藻属、鞘丝藻属、拟柱孢藻属和梅泽氏藻属菌株的基因组草图。卵形孢梅泽氏藻BLCC - F208、微囊藻属BLCC - F209、微囊藻属BLCC - F210、鞘丝藻属BLCC - F211、卵形孢梅泽氏藻BLCC - F215和拟柱孢藻BLCC - F218的全基因组鸟枪法测序项目已分别以登录号JBHFLK000000000、JBHFLL000000000、CP169647,、JBHFLM000000000、JBHFLN000000000和JBHFLO000000000存入GenBank。基于基因组分析，在不同的蓝藻菌株中鉴定出了几个与已知有毒和生物活性化合物基因簇具有不同程度相似性的生物合成基因簇（BCG）。