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[Isolation of neutral proteinase of ribosomes (cathepsin R)].

作者信息

Bylinkina V S

出版信息

Biokhimiia. 1986 Jan;51(1):65-9.

PMID:3955105
Abstract

A method for isolation of cathepsin R from rat liver ribosomes allowing for a 264-fold increase of specific activity is described. The purification procedure includes enzyme extraction from ribosomes with 2-4 M LiCl and two-step affinity chromatography on Sepharose with immobilized soy bean trypsin inhibitor and trypsin-Sepharose.

摘要

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