Xu Qiuting, Su Wei, Huang Cuilan, Zhong Haiyi, Huo Lini, Cai Jinyun, Li Peiyuan
Guangxi University of Chinese Medicine, Nanning, 530001, China.
Guangxi Key Laboratory of Natural Polymer Chemistry and Physics, Nanning Normal University, Nanning, 530001, China.
Adv Healthc Mater. 2025 Jan;14(3):e2403267. doi: 10.1002/adhm.202403267. Epub 2024 Nov 17.
As the misuse of antibiotics increases bacterial resistance, the treatment of infected wounds caused by bacteria encounters significant challenges. Conventional antimicrobial dressings often fall short in their ability to inhibit bacterial infections while simultaneously promoting wound healing. To address this issue, a polysaccharide self-healing hydrogel (CPP@PDA/Que3) wound dressing is successfully developed by incorporating quercetin and polydopamine nanoparticles into a carboxymethyl chitosan matrix. The dressing can be easily injected locally to create a protective barrier over the wound, effectively stopping bleeding and rapidly inhibiting inflammation. Furthermore, the CPP@PDA/Que3 hydrogel exhibits remarkable antioxidant and antibacterial properties, stemming from the combination of quercetin and near-infrared (NIR) photothermal therapy. It demonstrates the ability to eliminate 99.52% of Staphylococcus aureus and 99.39% of Escherichia coli in in vitro antibacterial experiments. Additionally, the in vivo wound healing experiment shows a healing rate of ≈97%. The experimental results indicate that under NIR laser (808 nm) irradiation, the polysaccharide-based hydrogel dressing significantly inhibits bacterial growth, reduces oxidative stress, expedites angiogenesis, and thereby accelerates the transition from inflammation to wound healing. In summary, the CPP@PDA/Que3 hydrogel exhibits significant potential as a wound dressing, providing a novel approach for clinically advancing the treatment of bacterial wounds.
随着抗生素的滥用增加了细菌耐药性，由细菌引起的感染伤口的治疗面临重大挑战。传统的抗菌敷料在抑制细菌感染同时促进伤口愈合的能力方面往往有所不足。为了解决这个问题，通过将槲皮素和聚多巴胺纳米颗粒掺入羧甲基壳聚糖基质中，成功开发了一种多糖自愈合水凝胶（CPP@PDA/Que3）伤口敷料。该敷料可以很容易地局部注射以在伤口上形成保护屏障，有效止血并迅速抑制炎症。此外，CPP@PDA/Que3水凝胶具有显著的抗氧化和抗菌性能，这源于槲皮素和近红外（NIR）光热疗法的结合。在体外抗菌实验中，它能够消除99.52%的金黄色葡萄球菌和99.39%的大肠杆菌。此外，体内伤口愈合实验显示愈合率约为97%。实验结果表明，在近红外激光（808nm）照射下，基于多糖的水凝胶敷料显著抑制细菌生长，降低氧化应激，加速血管生成，从而加速从炎症到伤口愈合的转变。总之，CPP@PDA/Que3水凝胶作为伤口敷料具有巨大潜力，为临床上推进细菌伤口的治疗提供了一种新方法。
