Akhlagh Alireza, Iraji Aida, Daneshi Seyyed Sajad, Kian Mehdi, Jamshidzadeh Akram, Zare Shahrokh, Tanideh Nader, Naseh Maryam, Mussin Nadiar M, Kurmanalina Madina A, Tamadon Amin
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Nat Prod Res. 2024 Nov 18:1-15. doi: 10.1080/14786419.2024.2429111.
Osteochondral defects (OCDs) in synovial joints are caused by trauma or inflammatory joint diseases, with no definitive treatment available. This study examined the effects of resveratrol and chondrocyte injections in a rat model of OCD. Twenty-four male Sprague-Dawley rats were divided into four groups: a control group, a resveratrol-only group (10 mg/kg), a chondrocyte-only group (1 × 10 cells), and a combined treatment group that received both treatments. After two months, the rats were euthanized, and their knee joints were analysed histologically and immunohistochemically. The results showed that the combined resveratrol and chondrocyte treatment significantly reduced fibrous tissue, increased cartilage tissue volume, improved cellular distribution, and enhanced the regularity of the articular surface. Collagen types I and II and proteoglycan levels were also elevated. These findings suggest that the combination of resveratrol and chondrocytes has a synergistic effect, promoting effective OCD repair in this rat model, offering potential for future therapeutic approaches.
滑膜关节中的骨软骨缺损（OCDs）由创伤或炎性关节疾病引起，目前尚无确切的治疗方法。本研究在OCD大鼠模型中考察了白藜芦醇和软骨细胞注射的效果。将24只雄性Sprague-Dawley大鼠分为四组：对照组、仅使用白藜芦醇组（10mg/kg）、仅使用软骨细胞组（1×10个细胞）以及接受两种治疗的联合治疗组。两个月后，对大鼠实施安乐死，并对其膝关节进行组织学和免疫组织化学分析。结果显示，白藜芦醇与软骨细胞联合治疗显著减少了纤维组织，增加了软骨组织体积，改善了细胞分布，并增强了关节表面的规则性。I型和II型胶原蛋白以及蛋白聚糖水平也有所升高。这些发现表明，白藜芦醇与软骨细胞的组合具有协同作用，可促进该大鼠模型中OCD的有效修复，为未来的治疗方法提供了潜力。
