Research Center of Nano Technology and Application Engineering, The First Dongguan Affiliated Hospital, School of Pharmacy, Guangdong Medical University, Dongguan, Guangdong, 523808, People's Republic of China.
Dongguan Biomedical Nano Engineering Technology Research Center, Guangdong Medical University, Dongguan, Guangdong, 523808, People's Republic of China.
Int J Nanomedicine. 2024 Nov 11;19:11671-11688. doi: 10.2147/IJN.S487683. eCollection 2024.
Melanoma is a highly aggressive and dangerous malignant skin tumor and there is an urgent need to develop effective therapeutic approaches against melanoma. The main objective of this study was to construct a multifunctional nanomedicine (GNR@PEG-Qu) to investigate its therapeutic effect on melanoma from the oxidative stress pathway.
First, the nanomedicine GNR@PEG-Qu was synthesized and characterized, and its photothermal and antioxidant properties were confirmed. In addition, in vivo imaging capabilities were observed. Finally, the tumor inhibitory effects of GNR@PEG-Qu in vivo and in vitro as well as its biosafety were observed.
GNR@PEG-Qu shows good photothermal and anti-oxidation properties. Following exposure to 1064 nm laser irradiation in the second near-infrared II (NIR-II) window, GNR@PEG-Qu shows anti-tumor ability through low-temperature photothermal therapy (PTT) adjuvant drug chemotherapy. GNR@PEG-Qu makes full use of the antioxidant capacity of quercetin, reduces ROS levels in melanoma, alleviates oxidative stress state, and achieves "oxidative stress avoidance" at the tumor site. Quercetin can also downregulate the expression of the heat shock protein Hsp70, which will improve the thermal sensitivity of the tumor site and enhance the efficacy of low-temperature PTT.
GNR@PEG-Qu nanoagent exhibits synergistic treatment and high tumor inhibition effects, which is a promising strategy developed to achieve oxidative stress avoidance and synergistic therapy of melanoma using quercetin (Qu)-coated gold nanorod (GNR@PEG).
黑色素瘤是一种高度侵袭性和危险的恶性皮肤肿瘤，因此迫切需要开发针对黑色素瘤的有效治疗方法。本研究的主要目的是构建一种多功能纳米药物（GNR@PEG-Qu），从氧化应激途径研究其对黑色素瘤的治疗作用。
首先合成并表征了纳米药物 GNR@PEG-Qu，并证实了其光热和抗氧化性能。此外，还观察了体内成像能力。最后，观察了 GNR@PEG-Qu 在体内和体外的肿瘤抑制作用及其生物安全性。
GNR@PEG-Qu 具有良好的光热和抗氧化性能。在近红外二区（NIR-II）1064nm 激光照射下，GNR@PEG-Qu 通过低温光热辅助药物化疗发挥抗肿瘤作用。GNR@PEG-Qu 充分利用槲皮素的抗氧化能力，降低黑色素瘤中的 ROS 水平，减轻氧化应激状态，实现在肿瘤部位的“氧化应激避免”。槲皮素还可以下调热休克蛋白 Hsp70 的表达，提高肿瘤部位的热敏感性，增强低温 PTT 的疗效。
GNR@PEG-Qu 纳米剂表现出协同治疗和高肿瘤抑制作用，这是一种有前途的策略，旨在使用槲皮素（Qu）包覆的金纳米棒（GNR@PEG）实现黑色素瘤的氧化应激避免和协同治疗。
