Juguelin H, Heape A, Boiron F, Cassagne C
Brain Res. 1986 Mar;390(2):249-52. doi: 10.1016/s0006-8993(86)80233-5.
The quantitative accumulation of neutral lipids during the period of myelination in the peripheral nervous system was studied in normal and trembler mouse sciatic nerves, between the ages of 5 and 27 days. Neutral lipids were resolved by high-performance thin-layer chromatography, using the solvent mixture hexane/diethyl ether/acetic acid (90:15:2, v/v/v). The lipids were quantitated, after copper acetate/phosphoric acid charring, by densitometric scanning, using an external standard technique. Cholesterol and triacylglycerols accumulated in normal nerves throughout the period studied, while cholesteryl esters were not observed at any age. In trembler nerves, the accumulation of cholesterol took place at a much lower rate than in normal nerves and this lipid was deficient from the earliest stages of development. Triacylglycerols were not significantly deficient in trembler nerves during the first 2-3 weeks, but, after the age of 18 days, their quantity diminished significantly. Cholesteryl esters were first detected in the mutant nerves at the age of 18 days. These results, in agreement with those of a previous developmental study of the polar lipids, are strongly in favour of the view that the trembler mutation directly induces a process of dysmyelination and that demyelination is a secondary event.
在5至27日龄的正常和震颤小鼠坐骨神经中，研究了外周神经系统髓鞘形成期间中性脂质的定量积累。使用己烷/乙醚/乙酸（90:15:2，v/v/v）混合溶剂，通过高效薄层色谱法分离中性脂质。脂质经醋酸铜/磷酸炭化后，采用外标技术通过光密度扫描进行定量。在所研究的整个期间，胆固醇和三酰甘油在正常神经中积累，而在任何年龄都未观察到胆固醇酯。在震颤神经中，胆固醇的积累速度比正常神经低得多，并且这种脂质从发育的最早阶段就缺乏。在最初的2至3周内，震颤神经中的三酰甘油没有明显缺乏，但在18日龄后，其数量显著减少。在18日龄时首次在突变神经中检测到胆固醇酯。这些结果与先前关于极性脂质的发育研究结果一致，强烈支持震颤突变直接诱导脱髓鞘过程且脱髓鞘是继发事件的观点。
