An experimental analysis of graph representation learning for Gene Ontology based protein function prediction.

Understanding protein function is crucial for deciphering biological systems and facilitating various biomedical applications. Computational methods for predicting Gene Ontology functions of proteins emerged in the 2000s to bridge the gap between the number of annotated proteins and the rapidly growing number of newly discovered amino acid sequences. Recently, there has been a surge in studies applying graph representation learning techniques to biological networks to enhance protein function prediction tools. In this review, we provide fundamental concepts in graph embedding algorithms. This study described graph representation learning methods for protein function prediction based on four principal data categories, namely PPI network, protein structure, Gene Ontology graph, and integrated graph. The commonly used approaches for each category were summarized and diagrammed, with the specific results of each method explained in detail. Finally, existing limitations and potential solutions were discussed, and directions for future research within the protein research community were suggested.