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鉴定源自海星的对C2C12肌管生长有活性的EPA/AA结合醚型磷脂酰胆碱。

Identification of the Active EPA/AA-Binding Ether-Type Phosphatidylcholine Derived from the Starfish for C2C12 Myotube Growth.

作者信息

Fukushima Aoi, Imamura Kyosuke, Takatani Naoki, Hosokawa Masashi, Beppu Fumiaki

机构信息

Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.

出版信息

ACS Omega. 2024 Oct 30;9(45):45564-45571. doi: 10.1021/acsomega.4c07865. eCollection 2024 Nov 12.

DOI:10.1021/acsomega.4c07865
PMID:39554463
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11561627/
Abstract

Concerns about nutritional approaches for promoting skeletal muscle mass and function have increased. This study assessed the effects of starfish-derived glycerophospholipids (PLs) (SPL), characterized by unique ether-linked subclasses, alkylacyl (Alk)- and alkenylacyl (Pls)-PL, on skeletal muscle function, focusing on myotube formation in C2C12 myoblasts. SPL was prepared via chloroform/methanol extraction from , followed by silica gel chromatography fractionation. Myoblasts were induced to differentiate with or without SPL treatment. On day 7 of differentiation, 50 μg/mL of SPL treatment increased myotube diameter. The phosphatidylcholine (PC) fraction (SPC) also enhanced myotube growth at 30 μg/mL. LC-MS/MS analysis indicated the most abundant PC molecular species in SPC were Alk- and Pls-PC with eicosapentaenoic acid and arachidonic acid. Treatment with 1--hexadecyl-2-arachidonoyl-PC, 1-1()-hexadecenyl-2-arachidonoyl-PC or 1--hexadecyl-2-eicosapentaenoyl-PC increased myotube diameter and myokine Il-15 mRNA expression. These results demonstrate a novel functionality of SPC and highlight the role of ether-type PC molecules in muscle function.

摘要

对促进骨骼肌质量和功能的营养方法的关注日益增加。本研究评估了海星来源的甘油磷脂（PLs）（SPL）对骨骼肌功能的影响，该甘油磷脂具有独特的醚键连接亚类，即烷基酰基（Alk）-和烯基酰基（Pls）-PL，重点研究了其对C2C12成肌细胞中肌管形成的影响。SPL通过氯仿/甲醇从……中提取制备，随后进行硅胶柱色谱分离。在有或没有SPL处理的情况下诱导成肌细胞分化。在分化第7天，50μg/mL的SPL处理增加了肌管直径。磷脂酰胆碱（PC）组分（SPC）在30μg/mL时也增强了肌管生长。液相色谱-串联质谱（LC-MS/MS）分析表明，SPC中最丰富的PC分子种类是含有二十碳五烯酸和花生四烯酸的Alk-PC和Pls-PC。用1-十六烷基-2-花生四烯酰基-PC、1-（1Z）-十六碳烯基-2-花生四烯酰基-PC或1-十六烷基-2-二十碳五烯酰基-PC处理可增加肌管直径和肌因子Il-15 mRNA表达。这些结果证明了SPC的一种新功能，并突出了醚型PC分子在肌肉功能中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe47/11561627/463c9f6fce29/ao4c07865_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe47/11561627/3770d1dfd998/ao4c07865_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe47/11561627/bff872e2a3b0/ao4c07865_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe47/11561627/3c00d5aa07f2/ao4c07865_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe47/11561627/46e2d0308b65/ao4c07865_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe47/11561627/463c9f6fce29/ao4c07865_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe47/11561627/3770d1dfd998/ao4c07865_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe47/11561627/bff872e2a3b0/ao4c07865_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe47/11561627/3c00d5aa07f2/ao4c07865_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe47/11561627/46e2d0308b65/ao4c07865_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe47/11561627/463c9f6fce29/ao4c07865_0005.jpg

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Sea cucumber ether-phospholipids improve hepatic steatosis and enhance hypothalamic autophagy in high-fat diet-fed mice.
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