长期酒精摄入及其戒断后大鼠肝细胞膜的适应性变化。

Adaptive changes in rat liver plasma membranes after chronic alcohol administration and its subsequent withdrawal.

作者信息

Lee H, Hosein E A

出版信息

Can J Physiol Pharmacol. 1986 Jan;64(1):85-92. doi: 10.1139/y86-013.

Abstract

Rat hepatic plasma membranes isolated after chronic alcohol feeding displayed a different buoyant density range with a significantly increased peak density value when spun isopycnically in a 30-50% sucrose (w/w) gradient. This change persisted up to 48 h of withdrawal from alcohol. Analysis of membrane lipids revealed certain significant alterations in the phospholipids as well as the fatty acyl composition in individual phospholipids of the experimental plasma membranes. During withdrawal of alcohol for 48 h, all the alcohol-induced changes in the phospholipids returned to normal. Most initial changes in fatty acids reverted to the control composition during this time, but new changes in fatty acyl distribution were also observed. These were interpreted to represent readaptation to the withdrawal of the alcohol. It is not established how long this readaptation period lasts.

摘要

慢性酒精喂养后分离得到的大鼠肝细胞膜，在30 - 50%（w/w）蔗糖梯度中进行等密度离心时，显示出不同的浮力密度范围，峰值密度值显著增加。这种变化在戒酒48小时内持续存在。对膜脂质的分析表明，实验性质膜的磷脂以及个别磷脂中的脂肪酰基组成存在某些显著变化。在戒酒48小时期间，所有酒精诱导的磷脂变化都恢复正常。在此期间，脂肪酸的大多数初始变化恢复到对照组成，但也观察到脂肪酰基分布的新变化。这些变化被解释为代表对戒酒的重新适应。目前尚未确定这种重新适应期持续多长时间。