Li Faji, Guo Can, Zhao Qi, Wen Weie, Zhai Shengnan, Cao Xinyou, Liu Cheng, Cheng Dungong, Guo Jun, Zi Yan, Liu Aifeng, Song Jianmin, Liu Jianjun, Liu Jindong, Li Haosheng
Crop Research Institute, National Engineering Laboratory for Wheat and Maize, National Key Laboratory of Wheat Improvement, Key Laboratory of Wheat Biology and Genetic Improvement in the Northern Yellow-Huai Rivers Valley of Ministry of Agriculture and Rural Affairs, Shandong Academy of Agricultural Sciences, Jinan, China.
Shangqiu Academy of Agriculture and Forestry Sciences, Shangqiu, China.
Front Plant Sci. 2024 Nov 1;15:1457437. doi: 10.3389/fpls.2024.1457437. eCollection 2024.
Powdery mildew (PM) poses an extreme threat to wheat yields and quality.[Methods] In this study, 262 recombinant inbred lines (RILs) of Doumai and Shi 4185 cross were used to map PM resistance genes across four environments. A high-density genetic linkage map of the Doumai/Shi 4185 RIL population was constructed using the wheat Illumina iSelect 90K single-nucleotide polymorphism (SNP) array.
In total, four stable quantitative trait loci (QTLs) for PM resistance, QPm.caas-2AS, QPm.caas-4AS, QPm.caas-4BL, and QPm.caas-6BS, were detected and explained 5.6%-15.6% of the phenotypic variances. Doumai contributed all the resistance alleles of QPm.caas-2AS, QPm.caas-4AS, QPm.ca as-4BL, and QPm.caas-6BS. Among these, QPm.caas-4AS and QPm.caas-6BS overlapped with the previously reported loci, whereas QPm.caas-2AS and QPm.caas-4BL are potentially novel. Additionally, six high-confidence genes encoding the NBS-LRR-like resistance protein, disease resistance protein family, and calcium/calmodulin-dependent serine/threonine-kinase were selected as the candidate genes for PM resistance. Three kompetitive allele-specific PCR (KASP) markers, Kasp_PMR_2AS for QPm.caas-2AS, Kasp_PMR_4BL for QPm.caas-4BL, and Kasp_PMR_6BS for QPm.caas-6BS, were developed, and their genetic effects were validated in a natural population including 100 cultivars.
These findings will offer valuable QTLs and available KASP markers to enhance wheat marker-assisted breeding for PM resistance.
白粉病对小麦产量和品质构成极大威胁。【方法】本研究利用豆麦与石4185杂交的262个重组自交系（RILs）在四个环境中定位白粉病抗性基因。使用小麦Illumina iSelect 90K单核苷酸多态性（SNP）芯片构建了豆麦/石4185 RIL群体的高密度遗传连锁图谱。
共检测到4个稳定的白粉病抗性数量性状位点（QTL），即QPm.caas - 2AS、QPm.caas - 4AS、QPm.caas - 4BL和QPm.caas - 6BS，它们解释了5.6% - 15.6%的表型变异。豆麦贡献了QPm.caas - 2AS、QPm.caas - 4AS、QPm.caas - 4BL和QPm.caas - 6BS的所有抗性等位基因。其中，QPm.caas - 4AS和QPm.caas - 6BS与先前报道的位点重叠，而QPm.caas - 2AS和QPm.caas - 4BL可能是新的。此外，选择了6个编码NBS - LRR样抗性蛋白、抗病蛋白家族以及钙/钙调蛋白依赖性丝氨酸/苏氨酸激酶的高可信度基因作为白粉病抗性的候选基因。开发了3个竞争性等位基因特异性PCR（KASP）标记，即用于QPm.caas - 2AS的Kasp_PMR_2AS、用于QPm.caas - 4BL的Kasp_PMR_4BL和用于QPm.caas - 6BS的Kasp_PMR_6BS，并在包括100个品种的自然群体中验证了它们的遗传效应。
这些发现将为加强小麦抗白粉病分子标记辅助育种提供有价值的QTL和可用的KASP标记。
