Li Yahui, Hu Jinghuang, Lin Huailong, Qiu Dan, Qu Yunfeng, Du Jiuyuan, Hou Lu, Ma Lin, Wu Qiuhong, Liu Zhiyong, Zhou Yijun, Li Hongjie
College of Life and Environmental Science, Minzu University of China, Beijing, China.
The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Plant Sci. 2024 May 8;15:1397274. doi: 10.3389/fpls.2024.1397274. eCollection 2024.
A recombinant inbred line (RIL) population derived from wheat landrace Qingxinmai and breeding line 041133 exhibited segregation in resistance to powdery mildew and stripe rust in five and three field tests, respectively. A 16K genotyping by target sequencing (GBTS) single-nucleotide polymorphism (SNP) array-based genetic linkage map was used to dissect the quantitative trait loci (QTLs) for disease resistance. Four and seven QTLs were identified for adult-plant resistance (APR) against powdery mildew and stripe rust. and on chromosomes 1B and 5A were responsible for the APR against powdery mildew in line 041133. , , , , , and detected on the five B-genome chromosomes of line 041133 conferred its APR to stripe rust. and were co-localized with the pleiotropic locus . A Kompetitive Allele Specific Polymorphic (KASP) marker was developed to trace . Four lines pyramiding six major disease resistance loci, , , , , , and , were developed. They displayed effective resistance against both powdery mildew and stripe rust at the seedling and adult-plant stages.
一个源自小麦地方品种清新麦和育种系041133的重组自交系（RIL）群体，在分别进行的5次和3次田间试验中，对白粉病和条锈病的抗性表现出分离。利用基于16K目标测序基因分型（GBTS）单核苷酸多态性（SNP）阵列构建的遗传连锁图谱，剖析抗病性的数量性状位点（QTL）。共鉴定出4个和7个针对成株期白粉病抗性（APR）和条锈病抗性的QTL。位于1B和5A染色体上的 和 对041133系的成株期白粉病抗性起作用。在041133系的5条B基因组染色体上检测到的 、 、 、 、 和 赋予其对条锈病的成株期抗性。 和 与多效性位点 共定位。开发了一个竞争性等位基因特异性多态性（KASP）标记 来追踪 。构建了4个聚合了6个主要抗病位点 、 、 、 、 和 的株系。它们在苗期和成株期均对白粉病和条锈病表现出有效的抗性。
