Han Chao, Chen Caiping, Lu Ning, Xue Li, Xing Dan, Wu Wanxin, Li Wang, Lu Xiang
Department of Breast Surgery, Affiliated Hospital of Jiaxing University (The First Hospital of Jiaxing), Jiaxing, China.
Department of Pathology, Affiliated Hospital of Jiaxing University (The First Hospital of Jiaxing), Jiaxing, China.
Clin Med Insights Oncol. 2024 Nov 16;18:11795549241298978. doi: 10.1177/11795549241298978. eCollection 2024.
Platelet-rich plasma (PRP) helps promote wound healing, but it is unclear whether it stimulates breast cancer cell proliferation, which restricts its application in breast cancer patients. This article explored the effect of PRP on breast cancer cell proliferation through preclinical experiments.
We cultivated MDA-MB-231 breast cancer cells with PRP in vitro. Subsequently, we employed Cell Counting Kit-8 (CCK-8) assays to assess their proliferation ability, wound healing assays to evaluate their migration ability, and Transwell assays to detect their invasion ability. Mouse breast cancer 4T1 cells were subcutaneously inoculated into nude mice. After tumor formation, PRP was injected around each tumor. Tumor size was measured. After 15 days, the tumors were surgically removed. Immunohistochemistry was used to detect key proteins involved in proliferation and apoptosis.
PRP inhibited the proliferation, migration, and invasion of MDA-MB-231 in vitro. After PRP was injected around tumors in nude mice, tumor growth slowed, Ki67 and phospho-histone H3 (pHH3) expression decreased, and Caspase 3 and Poly (adenosine diphosphate-ribose) polymerase 1 (PARP1) expression increased.
The PRP inhibits the proliferation of breast cancer MDA-MB-231 and 4T1 cells.
富血小板血浆（PRP）有助于促进伤口愈合，但尚不清楚它是否会刺激乳腺癌细胞增殖，这限制了其在乳腺癌患者中的应用。本文通过临床前实验探讨了PRP对乳腺癌细胞增殖的影响。
我们在体外使用PRP培养MDA-MB-231乳腺癌细胞。随后，我们采用细胞计数试剂盒-8（CCK-8）测定法评估其增殖能力，伤口愈合测定法评估其迁移能力，Transwell测定法检测其侵袭能力。将小鼠乳腺癌4T1细胞皮下接种到裸鼠体内。肿瘤形成后，在每个肿瘤周围注射PRP。测量肿瘤大小。15天后，手术切除肿瘤。采用免疫组织化学法检测参与增殖和凋亡的关键蛋白。
PRP在体外抑制MDA-MB-231的增殖、迁移和侵袭。在裸鼠肿瘤周围注射PRP后，肿瘤生长减缓，Ki67和磷酸化组蛋白H3（pHH3）表达降低，半胱天冬酶3和聚（二磷酸腺苷-核糖）聚合酶1（PARP1）表达增加。
PRP抑制乳腺癌MDA-MB-231和4T1细胞的增殖。
