Machado Caio C S, Cadeado Alegre N S, da Mota Yugo S N, Petruci João F S, Silva Sidnei G
Federal University of Uberlândia, Institute of Chemistry, 2121 João Naves de Ávila Avenue, Santa Mônica, Uberlândia, MG 38400-902, Brazil.
Anal Methods. 2024 Dec 12;16(48):8427-8436. doi: 10.1039/d4ay01831a.
Portable and affordable (spectro)photometers based on digital color sensors are gaining significance in analytical chemistry due to their compact design, cost-effectiveness and integration capabilities. Despite their inherent straight-forward operation, various digital light sensors with distinct performance and optical characteristics are available on the market. For instance, AS7341 and AS7262 are multi-channel color sensors that covers eight and six spectra regions, respectively, while TCS34725 operates using three channels (red, green, and blue). In this study, the analytical performance of 3D printed, reflectance-based (spectro)photometers employing different digital color sensors was evaluated. Spectra resolution, signal gain and LED current were systematically evaluated to ensure the best performance for each sensor. The sensors' analytical performance was obtained by quantifying different dyes in aqueous solutions and sunset yellow in beverages as a proof-of-concept. All three sensors presented sub-millimolar detection with excellent linear coefficient ( > 0,99) and sensitivity comparable to a conventional spectrophotometer. In the quantification of sunset yellow ( = 480 nm) in the presence of tartrazine ( = 430 nm), the AS sensors showed enhanced sensitivity and linearity, minimizing the spectral overlapping. Their versatility and cost-effectiveness make them ideal for real-time, portable applications. Furthermore, their compatibility with microcontrollers and capability for wireless data transmission enhance their utility across diverse analytical environments.
基于数字颜色传感器的便携式且价格实惠的（光谱）光度计，因其紧凑的设计、成本效益和集成能力，在分析化学中日益重要。尽管其操作本身简单直接，但市场上有各种具有不同性能和光学特性的数字光传感器。例如，AS7341和AS7262是多通道颜色传感器，分别覆盖八个和六个光谱区域，而TCS34725使用三个通道（红色、绿色和蓝色）运行。在本研究中，评估了采用不同数字颜色传感器的3D打印、基于反射率的（光谱）光度计的分析性能。系统地评估了光谱分辨率、信号增益和LED电流，以确保每个传感器的最佳性能。通过对水溶液中的不同染料和饮料中的日落黄进行定量，作为概念验证，获得了传感器的分析性能。所有三种传感器都呈现出亚毫摩尔级检测，具有出色的线性系数（>0.99），且灵敏度与传统分光光度计相当。在柠檬黄（λ = 430 nm）存在的情况下对日落黄（λ = 480 nm）进行定量时，AS传感器显示出更高的灵敏度和线性度，最大限度地减少了光谱重叠。它们的多功能性和成本效益使其成为实时、便携式应用的理想选择。此外，它们与微控制器的兼容性以及无线数据传输能力增强了它们在各种分析环境中的实用性。
