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一种从粪便中分离沙门氏菌的快速方法(作者译)

[A rapid method for the isolation of Salmonellae from faeces (author's transl)].

作者信息

Ruffini G, Santagata L, Grechi G

出版信息

Quad Sclavo Diagn. 1978 Dec;14(4):494-9.

PMID:395562
Abstract

A rapid and simple method for detecting Salmonellae in faeces was developed. It was based on the motility of Salmonellae through a semi-solid medium in a U-tube. The migration of other intestinal bacteria was retarded by means of a magnesium salt concentration. 474 stool specimens were examined for Salmonellae in parallel with the usual isolation method. A total of 39 Salmonella strains were isolated, 37 (94.9%) by means of the selective migration procedure, 26 (66.7%) when the routine method was applied. Two strains of patients in chemo-therapy were missed by the rapid method, which was moreover inefficient in detecting S. typhi, due to its slow motility. However, the rapid method is recommended in addition to conventional mediums in screening of carriers for its higher frequency of positive isolations compared with multistep procedure (+27.3%).

摘要

开发了一种快速简便的粪便中沙门氏菌检测方法。该方法基于沙门氏菌在U型管半固体培养基中的运动性。通过镁盐浓度抑制其他肠道细菌的迁移。与常规分离方法平行检测了474份粪便标本中的沙门氏菌。共分离出39株沙门氏菌菌株,通过选择性迁移程序分离出37株(94.9%),采用常规方法时分离出26株(66.7%)。快速方法漏检了2例化疗患者的菌株,此外,由于伤寒沙门氏菌运动缓慢,该方法在检测伤寒沙门氏菌时效率低下。然而,与多步骤程序相比,快速方法在携带者筛查中阳性分离频率更高(+27.3%),因此除传统培养基外,推荐使用该快速方法。

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