College of Plant Protection, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Weigang Road 1, Nanjing, Jiangsu 210095, China.
Centre for Ecology and Conservation, University of Exeter, Penryn TR10 9FE, U.K.
J Agric Food Chem. 2024 Nov 27;72(47):26475-26490. doi: 10.1021/acs.jafc.4c06839. Epub 2024 Nov 18.
The emergence of insecticide resistance in the rice stem borer, , is a growing threat to the sustainable control of this important insect crop pest. Thus, monitoring of populations for insecticide resistance and characterization of the underlying genetic mechanisms is essential to inform rational control decisions and the development of resistance management strategies. Here, we monitored 126 field populations from China for resistance evolution to four major insecticides: 53 for chlorantraniliprole, 50 for abamectin, 74 for triazophos, and 76 for spinetoram. Moderate to high levels of resistance were observed to all four insecticides. Investigation of the underlying resistance mechanisms revealed multiple mutations in the ryanodine receptor (RyR) and acetylcholinesterase 1 (AChE1), leading to target-site resistance to chlorantraniliprole and triazophos, respectively. In contrast, the absence of mutations in the glutamate-gated chloride channel (GluCl) and α6 nicotinic acetylcholine receptor (nAChR α6) subunit suggested that nontarget site mechanisms contribute to the multiple-insecticide resistance phenotypes observed in . In this regard, we revealed overexpression of the uridine 5'-diphospho-glycosyltransferase and cytochrome P450 in field populations. Functional characterization using transgenic demonstrated that UGT33AF1 confers resistance against multiple insecticides , whereas CYP6AB45 does not appear to contribute to resistance. Collectively, our findings reveal the current status of resistance of to insecticides in China and uncover a diverse profile of resistance mechanisms in this species. These findings provide a foundation for the development of sustainable strategies to effectively manage and control this pest.
稻纵卷叶螟对杀虫剂的抗药性的出现，对这种重要的作物害虫的可持续控制构成了日益严重的威胁。因此，监测种群对杀虫剂的抗药性和鉴定其潜在的遗传机制对于合理的控制决策和抗药性管理策略的制定至关重要。在这里，我们监测了来自中国的 126 个田间种群对四种主要杀虫剂的抗药性演变：53 个种群对氯虫苯甲酰胺，50 个种群对阿维菌素，74 个种群对三唑磷，76 个种群对乙基多杀菌素。所有四种杀虫剂均观察到中等至高水平的抗性。对潜在的抗药性机制的研究表明，在 Ryanodine 受体（RyR）和乙酰胆碱酯酶 1（AChE1）中存在多种突变，分别导致对氯虫苯甲酰胺和三唑磷的靶标抗性。相比之下，在谷氨酸门控氯离子通道（GluCl）和α6 烟碱型乙酰胆碱受体（nAChRα6）亚基中没有发现突变，这表明非靶标机制对观察到的 多杀虫剂抗性表型有贡献。在这方面，我们揭示了 田间种群中尿嘧啶 5'-二磷酸糖基转移酶和细胞色素 P450 的过度表达。使用转基因的功能表征表明，UGT33AF1 赋予对多种杀虫剂的抗性，而 CYP6AB45 似乎对抗性没有贡献。总的来说，我们的研究结果揭示了中国稻纵卷叶螟对杀虫剂的抗药性现状，并揭示了该物种中多种抗药性机制的多样性。这些发现为制定可持续的策略来有效管理和控制这种害虫提供了基础。
