Wang Shuai, Huang Jing-Mei, Guo Fang-Rui, Liu Chong, Xie Yuan, Qiao Song-Tao, Chen Yun-Xiao, Wu Shun-Fan, Bass Chris, Gao Cong-Fen
College of Plant Protection, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Weigang Road 1, Nanjing, Jiangsu 210095, China.
College of Biological & Agricultural Sciences, Honghe University, Dongjiao Xuefu Road, Mengzi, Yunnan 661199, China.
J Agric Food Chem. 2024 Dec 4;72(48):26943-26956. doi: 10.1021/acs.jafc.4c09254. Epub 2024 Nov 19.
Understanding the role of flavin-containing monooxygenases (FMOs) in the genetic mechanisms of insecticide resistance is essential for developing effective management strategies against the rice stem borer, . In this study, we identified five FMO genes in , examined their expression patterns, and revealed overexpression of and in field populations resistant to multiple insecticides, including chlorantraniliprole and spinetoram. Functional characterization using transgenic indicated that FMO3B and FMO3C do not confer resistance to abamectin or methoxyfenozide but do mediate resistance to chlorantraniliprole and spinetoram. Knockdown of and increased sensitivity to these insecticides in . Molecular docking studies indicated direct binding of chlorantraniliprole and spinetoram to these FMOs, underscoring their role in metabolic resistance. These findings indicate that FMOs are key enzymes in the metabolic resistance of to chlorantraniliprole and spinetoram, enhancing our understanding of insecticide resistance and aiding the development of management strategies.
了解含黄素单加氧酶（FMOs）在杀虫剂抗性遗传机制中的作用对于制定针对水稻螟虫的有效管理策略至关重要。在本研究中，我们在[具体物种]中鉴定出五个FMO基因，检测了它们的表达模式，并揭示了在对包括氯虫苯甲酰胺和多杀霉素在内的多种杀虫剂具有抗性的田间种群中[具体基因]的过表达。使用转基因[具体物种]进行的功能表征表明，FMO3B和FMO3C不赋予对阿维菌素或甲氧虫酰肼的抗性，但确实介导对氯虫苯甲酰胺和多杀霉素的抗性。在[具体物种]中敲低[具体基因]会增加对这些杀虫剂的敏感性。分子对接研究表明氯虫苯甲酰胺和多杀霉素与这些FMOs直接结合，突出了它们在代谢抗性中的作用。这些发现表明FMOs是[具体物种]对氯虫苯甲酰胺和多杀霉素代谢抗性的关键酶，增强了我们对杀虫剂抗性的理解并有助于管理策略的制定。
