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比较转录组学和激素分析揭示了水杉不定根形成的潜在调控网络。

Comparative transcriptomic and hormonal analyses reveal potential regulation networks of adventitious root formation in Metasequoia glyptostroboides Hu et Cheng.

机构信息

Guangdong Provincial Key Laboratory of Applied Botany, South China, Botanical Garden , Chinese Academy of Sciences, Guangzhou, 510650, China.

University of the Chinese Academy of Sciences, Beijing, 100039, China.

出版信息

BMC Genomics. 2024 Nov 18;25(1):1098. doi: 10.1186/s12864-024-10989-6.

DOI:10.1186/s12864-024-10989-6
PMID:39558286
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11572361/
Abstract

BACKGROUND

The extract from Metasequoia glyptostroboides Hu et Cheng, a rare and endangered species native to China, exhibits numerous biological and pharmacological activities. The species is recalcitrant to rooting during micropropagation, a challenge that has yet to be resolved. In this study, transcriptomic and hormonal analyses were conducted to appreciate the molecular mechanism of adventitious root (AR) formation in optimized rooting conditions.

RESULTS

The use of 2/5-strength Woody Plant Medium (WPM) significantly promoted AR formation of M. glyptostroboides shoots while the content of endogenous auxin, cytokinins and gibberellins (GAs) varied at different stages of AR formation. Transcriptomic analysis showed the significant up- or down-regulation of differentially expressed genes (DEGs) associated with plant hormone signal transduction and the phenylpropanoid biosynthesis pathway in response to 2/5-strength WPM. DEGs related to the biosynthesis of indole-3-acetic acid, cytokinins and GAs were identified. Transcript factors involved in 13 families were also revealed. A weighted gene co-expression network analysis indicated a strong correlation between hormones and genes involved in plant hormone signal transduction and the phenylpropanoid biosynthetic pathway.

CONCLUSIONS

These results indicate that the AR-promoting potential of 2/5-strength WPM in M. glyptostroboides was due to complex interactions between hormones and the expression of genes related to plant hormone signal transduction and the phenylpropanoid biosynthetic pathway.

摘要

背景

中国特有的珍稀濒危物种柏科柏木属植物珙桐，其提取物具有多种生物和药理活性。该物种在微繁殖过程中生根困难，这一挑战尚未得到解决。本研究通过转录组和激素分析，探讨了优化生根条件下不定根（AR）形成的分子机制。

结果

使用 2/5 强度木本植物培养基（WPM）显著促进了珙桐芽的不定根形成，而内源生长素、细胞分裂素和赤霉素（GAs）的含量在不定根形成的不同阶段有所变化。转录组分析表明，与植物激素信号转导和苯丙烷生物合成途径相关的差异表达基因（DEGs）在响应 2/5 强度 WPM 时显著上调或下调。鉴定出与吲哚-3-乙酸、细胞分裂素和 GAs 生物合成相关的 DEGs。还揭示了涉及 13 个家族的转录因子。加权基因共表达网络分析表明，激素与参与植物激素信号转导和苯丙烷生物合成途径的基因之间存在很强的相关性。

结论

这些结果表明，2/5 强度 WPM 在珙桐中的促根潜力是由于激素与参与植物激素信号转导和苯丙烷生物合成途径的基因之间的复杂相互作用所致。

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