Cooke R R, McIntosh J E
Clin Chim Acta. 1986 Feb 15;154(3):171-9. doi: 10.1016/0009-8981(86)90028-8.
The advantages are illustrated of analyzing breast cancer estrogen and progesterone receptor assays by fitting a receptor-ligand binding model directly to experimental data, rather than using the common graphical transformation known as the 'Scatchard plot'. Analysis of laboratory experiments and the use of simulation show that while both methods give equivalent results, the direct approach has definite advantages. In particular, results are more reliable in the face of increasing experimental error, it is possible to quantify non-specific binding from the primary data rather than having to measure it separately, and the methodology readily lends itself to optimizing the design of experiments.
通过直接将受体-配体结合模型拟合到实验数据来分析乳腺癌雌激素和孕激素受体测定的优势得以体现，而不是使用被称为“Scatchard图”的常见图形转换方法。对实验室实验的分析以及模拟的运用表明，虽然两种方法得出的结果相当，但直接法具有明确的优势。特别是，面对不断增加的实验误差时结果更可靠，能够从原始数据中对非特异性结合进行量化，而不必单独测量，并且该方法很容易用于优化实验设计。
