Wang Hongfeng, Zhuang Yuli, Fu Siyi, Shen Yuxuan, Qian Huijuan, Yan Xiaoqiao, Ge Jingyan
Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
Anal Chem. 2024 Dec 3;96(48):18939-18945. doi: 10.1021/acs.analchem.4c02307. Epub 2024 Nov 19.
β-Galactosidase (β-gal) has emerged as a pivotal biomarker in primary ovarian cancer. Despite the existence of numerous fluorescent probes for β-gal activity detection, quinone methide-based immobilizing probes were shown to avoid rapid diffusion of the activated fluorophore and improve the resolution. However, the synthesis of these fluorophores, particularly near-infrared fluorophores, still exhibits lower efficiency. In this study, we introduce modular and rapidly assembled self-immobilizing fluorogenic probes, capitalizing on the proximity labeling properties of quinone methide (QM). Compared to conventional fluorescent probes, these new probes not only exhibit a fluorogenic response but also achieve permanent retention, demonstrating improved detection sensitivity, particularly after cell fixation and in vivo animal model studies. This straightforward synthesis approach holds promise for broader applications in detecting other analytes.
β-半乳糖苷酶（β-gal）已成为原发性卵巢癌中的关键生物标志物。尽管存在众多用于检测β-gal活性的荧光探针，但基于醌甲基化物的固定探针已被证明可避免活化荧光团的快速扩散并提高分辨率。然而，这些荧光团的合成，尤其是近红外荧光团的合成，效率仍然较低。在本研究中，我们利用醌甲基化物（QM）的邻近标记特性，引入了模块化且快速组装的自固定荧光探针。与传统荧光探针相比，这些新探针不仅表现出荧光响应，还实现了永久保留，显示出更高的检测灵敏度，特别是在细胞固定后以及体内动物模型研究中。这种简单的合成方法有望在检测其他分析物方面得到更广泛的应用。
