Zhao Chunyan, Song Chang, Yang Shixiong, Huang Aichun, Lin Yanrong, Xu Chaoyan, Wei Xiaoying, Zeng Chunmei, Lan Yiyi, Luo Xiaoyang, Zhu Qingdong
Department of Tuberculosis, The Fourth People's Hospital of Nanning, Nanning, China; Guangxi Medical University, Nanning, China.
Administrative Office, The Fourth People's Hospital of Nanning, Nanning, China.
Diagn Microbiol Infect Dis. 2025 Feb;111(2):116593. doi: 10.1016/j.diagmicrobio.2024.116593. Epub 2024 Nov 12.
To investigate the diagnostic efficacy of nanopore sequencing technology in tuberculous meningitis (TBM).
Cerebrospinal fluid samples were collected from patients for acid-fast staining microscopy, Mycobacterium tuberculosis solid culture, DNA detection, and nanopore sequencing. Lastly, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and area under the receiver operating characteristic curve (AUC) were calculated and compared among these detection methods.
In this study, 30 TBM patients and 18 non-TBM patients were included. Nanopore sequencing showed higher sensitivity (43.30 %) and AUC (0.661) compared to the other methods. Combining nanopore sequencing and imaging achieved the highest diagnostic performance with sensitivity (60.00 %), specificity (88.90 %), PPV (90.00 %), NPV (57.10 %), and AUC (0.744).
Nanopore sequencing demonstrated superior diagnostic accuracy for TBM, outperforming acid-fast staining, Mycobacterium tuberculosis culture, and DNA detection. When combined with imaging, nanopore sequencing significantly enhanced diagnostic sensitivity and accuracy for TBM.
探讨纳米孔测序技术在结核性脑膜炎（TBM）诊断中的效能。
收集患者的脑脊液样本进行抗酸染色显微镜检查、结核分枝杆菌固体培养、DNA检测和纳米孔测序。最后，计算并比较这些检测方法的敏感性、特异性、阳性预测值（PPV）、阴性预测值（NPV）以及受试者工作特征曲线下面积（AUC）。
本研究纳入了30例TBM患者和18例非TBM患者。与其他方法相比，纳米孔测序显示出更高的敏感性（43.30%）和AUC（0.661）。纳米孔测序与影像学检查相结合，诊断性能最佳，敏感性为60.00%，特异性为88.90%，PPV为90.00%，NPV为57.10%，AUC为0.744。
纳米孔测序对TBM显示出卓越的诊断准确性，优于抗酸染色、结核分枝杆菌培养和DNA检测。与影像学检查相结合时，纳米孔测序显著提高了TBM的诊断敏感性和准确性。
