Department of Zoology, Mizoram University, Aizawl, Mizoram 796 004, India.
Department of Zoology, University of Lucknow, Uttar Pradesh 226 007, India.
Ann Parasitol. 2024;70(1):35-40. doi: 10.17420/ap7001.519.
Dactylogyrus pharyngocephalus is a dactylogyrid monogenean parasite originally described by Kulkarni, 1970 from tank goby Glossogobius giuris (Hamilton, 1822) in South India. The species has not been recorded since then. Fresh specimens of D. pharyngocephalus were collected from northeast India and examined morpho-molecularly. Temporary (glycerine mounted) and permanent (stained with Gomori's trichrome and DPX mounted) slides were made and photographed. Primer set C1 and D2 was employed to amplify a fragment of the 28S rRNA gene. The resulting nucleotide sequences, when examined by the BLAST tool, did not show 100% homology with any of the sequences deposited in GenBank. Based on new morpho-molecular data, the description of D. pharyngocephalus is revised, including the first description of its ventral bar.
菲律宾副口吸虫是一种原本由库尔卡尼于 1970 年在印度南部从黄纹拟鲈(Hamilton,1822)中描述的副口科单殖吸虫。自那时以来,该物种一直没有被记录。从印度东北部采集到新鲜的菲律宾副口吸虫标本,并进行了形态分子学检查。制作了临时(甘油装片)和永久(用 Gomori 三色染色和 DPX 装片)载玻片,并进行了拍照。使用引物对 C1 和 D2 扩增了 28S rRNA 基因的一个片段。通过 BLAST 工具检查所得核苷酸序列,与 GenBank 中保存的任何序列均未显示 100%的同源性。基于新的形态分子数据,对菲律宾副口吸虫的描述进行了修订,包括其腹肋的首次描述。