Ameliorative Effects of Pearl Millet ( L.) Against Hydrogen Peroxide Induced Cognitive Impairment and Oxidative Stress in Rats.

Pearl millet (PM) ( L.) contains a wide variety of bioactive compounds, such as polyphenols, mostly flavonoids and phenolic acids. In the present study, we investigated the effects of PM activity against hydrogen peroxide (HO)-induced behavior impairment and oxidative damage in rats. The rats were divided into four groups based on the treatments they received over 30 days: Control, HO, PM + HO, and PM. The phytochemical screening, total polyphenols content (TFC), and total flavonoid content (TFC) were determined using colorimetric analysis. All animals were subjected to behavioral test (elevated plus maze test). Thereafter, oxidative stress response (malondialdehyde [MDA], HO, and Thiol groups [-SH]) contents and antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) were estimated in brain, liver, and kidney tissues. We evaluated the levels of liver enzymes, such as alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT). Our investigation showed that PM is rich in total phenolic content and TFC and exhibited an important antioxidant activity. , we first found that HO-treated rat exhibited anxiogenic behavior in the elevated plus maze test and the genesis of oxidative stress in the brain, liver, and kidney was measured by an increase of MDA and antioxidant enzyme activity depletion, such as SOD and CAT. Moreover, HO increased levels of liver enzymes (ALAT and ASAT). Pearl Mille administration improved emotional behavior impairments and significantly reversed HO-induced biochemical alterations. Thus, we suggest that the Pearl Mille may have an anxiolytic-like effect and prevent biochemical disorders associated from the oxidative stress (HO), confirming its potential therapeutic capability.