Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé, Cameroon.
Department of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Biomed Res Int. 2018 Nov 25;2018:4907648. doi: 10.1155/2018/4907648. eCollection 2018.
Medicinal plants are efficient modulators of oxidative stress associated with diabetes mellitus. This study evaluated the cardio-, reno-, and hepato-antioxidant status of hydroethanolic extract of on streptozotocin-intoxicated diabetic rats. Experimental animals were daily administered with hydroethanolic extract of by oral intubation for eight weeks (60 days), after which the levels of catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), and lipid peroxidation marker (MDA) were evaluated in the heart, liver, and kidney homogenates. Plasma biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total protein, creatinine, and urea were determined. Meanwhile, parts of the heart, kidneys, and liver were histopathologically examined. Streptozotocin administration induced toxicity in the cardiac, hepatic, and renal tissues by stimulating significant increases (p<0.05) in the levels of CAT and SOD, GSH, and MDA. Similarly, significant increases (P<0.05) in the levels of ALT, AST, urea, and total protein were observed in streptozotocin treated rats, whereas decreases were observed in the levels of ALP, LDH, and creatinine. Following the treatments with hydroethanolic extract prevented the effect of streptozotocin by maintaining the tissue antioxidant status (CAT, SOD, GSH, and MDA) and the plasma biochemical parameters (AST, ALT, ALP, LDH, creatinine, and urea) towards the normal ranges. The histopathological examination revealed hepatovascular congestion and leucocyte infiltration as well as renovascular congestion, glomerulosclerosis, and tubular clarification in the untreated diabetic control and their absence in the group of animals treated with a high dose of extract. The findings of the present investigation suggest that possesses antioxidant activities capable of regulating drug induced tissue damage.
药用植物是与糖尿病相关的氧化应激的有效调节剂。本研究评估了水醇提取物对链脲佐菌素中毒糖尿病大鼠的心、肾和肝抗氧化状态。实验动物通过口服插管每天给予水醇提取物 8 周（60 天），然后评估心脏、肝脏和肾脏匀浆中的过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）和脂质过氧化标志物（MDA）的水平。测定血浆生化参数如天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）、总蛋白、肌酐和尿素。同时，对心脏、肾脏和肝脏的部分组织进行了组织病理学检查。链脲佐菌素给药通过刺激 CAT 和 SOD、GSH 和 MDA 水平的显著增加（p<0.05）在心脏、肝脏和肾脏组织中引起毒性。同样，在链脲佐菌素处理的大鼠中观察到 ALT、AST、尿素和总蛋白水平的显著增加（P<0.05），而 ALP、LDH 和肌酐水平则下降。用 水醇提取物处理后，通过维持组织抗氧化状态（CAT、SOD、GSH 和 MDA）和血浆生化参数（AST、ALT、ALP、LDH、肌酐和尿素）在正常范围内，防止了链脲佐菌素的作用。组织病理学检查显示未经处理的糖尿病对照组存在肝血管充血和白细胞浸润以及肾血管充血、肾小球硬化和管状澄清，而用高剂量 提取物处理的动物组则不存在这些情况。本研究的结果表明， 具有抗氧化活性，能够调节药物引起的组织损伤。
