心肌细胞特异性 FoxO1 敲除小鼠作为评估心脏肥大的工具以及 Cre-loxP 的关键实验注意事项
Cardiomyocyte-Specific FoxO1 Knockout Mice as Tools to Assess Cardiac Hypertrophy and Key Experimental Considerations Using Cre-loxP.
机构信息
Department of Anatomy and Physiology, The University of Melbourne, Parkville, VIC, Australia.
Baker Department of Cardiometabolic Health, The University of Melbourne, Parkville, VIC, Australia.
出版信息
Methods Mol Biol. 2025;2871:69-81. doi: 10.1007/978-1-0716-4217-7_7.
The generation of tissue-specific mouse models has provided a powerful strategy to understand the role of genes in specific tissues/cells of interest under control/basal conditions and in response to physiological and pathological stimuli. Here we describe the generation of cardiomyocyte-specific FoxO1 knockout mice using Cre-loxP technology to examine the role of FoxO1 for the induction of heart enlargement (cardiac hypertrophy) in settings of health and disease. We highlight breeding strategies for generating tissue-specific mouse models and key experimental considerations during characterization.
组织特异性小鼠模型的产生为理解基因在特定组织/细胞中的作用提供了一种强大的策略，这些作用可在控制/基础条件下以及对生理和病理刺激的反应中观察到。本文中，我们使用 Cre-loxP 技术生成了心肌细胞特异性 FoxO1 敲除小鼠，以研究 FoxO1 在健康和疾病状态下诱导心脏增大（心肌肥厚）中的作用。我们重点介绍了生成组织特异性小鼠模型的繁殖策略和在特征鉴定过程中的关键实验注意事项。
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