Molecular and Cellular Biology, University of Arizona, Tucson, AZ, USA.
University of Arizona Cancer Center, Tucson, AZ, USA.
Methods Mol Biol. 2025;2871:131-143. doi: 10.1007/978-1-0716-4217-7_12.
FOXO transcription factors respond to a number of different stresses by shuttling from the cytoplasm to the nucleus where they upregulate hundreds of target genes with diverse cellular functions. The cellular consequences of FOXO activation are both stress and cell-type specific. Recent evidence suggests that one way in which FOXO dictates stress-specific outcomes is through distinct nuclear/cytoplasmic shuttling dynamics. Here we outline methods for measuring FOXO nuclear shuttling dynamics using fluorescence-based reporters.
FOXO 转录因子通过从细胞质到细胞核的穿梭对许多不同的应激作出反应，在细胞核中它们上调具有多种细胞功能的数百个靶基因。FOXO 激活的细胞后果既有应激特异性又有细胞类型特异性。最近的证据表明，FOXO 决定应激特异性结果的一种方式是通过独特的核/细胞质穿梭动力学。在这里，我们概述了使用基于荧光的报告器测量 FOXO 核穿梭动力学的方法。
