Liu Yanguo, Wu Yanfang, He Shujie
Department of Hepatobiliary and Pancreatic Surgery, East Hospital of Yantai Mountain Hospital, Yantai 264000, Shandong Province, China.
Department of Hepatobiliary and Pancreatic Surgery, East Hospital of Yantai Mountain Hospital, Yantai 264000, Shandong Province, China.
Hum Immunol. 2024 Nov;85(6):111173. doi: 10.1016/j.humimm.2024.111173. Epub 2024 Nov 20.
This study was to investigate the clinical value of microRNA (miR)-130a in acute liver failure (ALF).
ALF patients (n = 120, ALF group) and 106 healthy subjects (control group) were enrolled. Serum was collected to detect alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TBIL) by automatic biochemical analyzer, and miR-130a by real-time fluorescence quantitative PCR. According to the Child-Pugh score, ALF patients could be divided into grades A, B, and C, and levels of ALT, AST, TBIL, and miR-130a in each grade were observed. Pearson correlation coefficient method was employed to analyze the correlation between miR-130a and Child-Pugh scores and liver function indices. ALF patients were divided into high-low miR-130a expression groups, and poor prognoses were observed. The influence of miR-130a on prognosis was analyzed by Kaplan-Meier curve, and the prognostic value of miR-130a was analyzed by the ROC curve.
miR-130a, ALT, AST, and TBIL were increased in the ALF group. miR-130a, ALT, AST, and TBIL increased with the increase of the Child-Pugh grade. miR-130a levels were positively correlated with ALT, AST, and TBIL levels. The incidence of poor prognoses was 58.33% in the miR-130a high expression group and 30% in the miR-130a low expression group. The prognosis of the miR-130a low expression group was better than that of the miR-130a high expression group, and miR-130a had predictive value for the prognosis of ALF patients.
miR-130a is increased in ALF, and it has high value for both diagnosis and prognosis in ALF patients, and patients with high levels of miR-130a have a poor prognosis.
本研究旨在探讨微小RNA（miR）-130a在急性肝衰竭（ALF）中的临床价值。
纳入ALF患者120例（ALF组）和106例健康受试者（对照组）。采集血清，用自动生化分析仪检测丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和总胆红素（TBIL），用实时荧光定量PCR检测miR-130a。根据Child-Pugh评分，将ALF患者分为A、B、C级，观察各级患者的ALT、AST、TBIL和miR-130a水平。采用Pearson相关系数法分析miR-130a与Child-Pugh评分及肝功能指标之间的相关性。将ALF患者分为miR-130a高表达组和低表达组，观察不良预后情况。采用Kaplan-Meier曲线分析miR-130a对预后的影响，采用ROC曲线分析miR-130a的预后价值。
ALF组miR-130a、ALT、AST和TBIL升高。miR-130a、ALT、AST和TBIL随Child-Pugh分级升高而升高。miR-130a水平与ALT、AST和TBIL水平呈正相关。miR-130a高表达组不良预后发生率为58.33%，miR-130a低表达组为30%。miR-130a低表达组预后优于高表达组，miR-130a对ALF患者预后有预测价值。
ALF患者miR-130a升高，对ALF患者的诊断和预后均有较高价值，miR-130a水平高的患者预后较差。
