Martins Artur J, Perdigão Lara, Gonçalves Catarina, Amado Isabel R, Abreu Cristiano S, Vicente António A, Cunha Rosiane L, Pastrana Lorenzo M, Cerqueira Miguel A
International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga s/n, 4715-330 Braga, Portugal.
International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga s/n, 4715-330 Braga, Portugal; Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Food Chem. 2025 Feb 15;465(Pt 2):142085. doi: 10.1016/j.foodchem.2024.142085. Epub 2024 Nov 16.
Composition and structure of oleogels significantly influence their digestive behaviour, impacting triacylglycerol breakdown and the bioavailability of incorporated compounds. Texture profile analysis showed that sterol-based oleogels (STOs) exhibited 20 times higher hardness than beeswax-based oleogels (BWOs), which showed stronger cohesion due to elasticity sustained by adhesive forces. Tribological assessments revealed similar initial coefficients of friction (COF) for both oleogels. However, fluctuations were observed in BWOs and a gradual decrease in STOs over time, enhancing lubrication, while BWOs recorded higher adhesion. These findings provide insights into their distinct digestive behaviour, with both oleogels undergoing structural disintegration and STOs displaying a higher lipolysis degree. Non-cytotoxicity was confirmed under Caco-2 cells. β-carotene bioaccessibility was influenced by the oleogels' structural modification and values of 4.0 ± 0.7 % for STOs and 2.6 ± 1.1 % for BWOs were recorded. Results highlight the need to optimize formulations to improve bioactive's bioavailability, emphasizing the role of structured gels in modulating digestion dynamics.
油凝胶的组成和结构显著影响其消化行为，影响三酰甘油的分解以及所含化合物的生物利用度。质地剖面分析表明，基于甾醇的油凝胶（STOs）的硬度比基于蜂蜡的油凝胶（BWOs）高20倍，由于粘合力维持的弹性，BWOs表现出更强的内聚力。摩擦学评估显示两种油凝胶的初始摩擦系数（COF）相似。然而，观察到BWOs的摩擦系数有波动，而STOs随时间逐渐降低，润滑性增强，同时BWOs的粘附力更高。这些发现为它们不同的消化行为提供了见解，两种油凝胶都经历了结构解体，且STOs表现出更高的脂解程度。在Caco-2细胞下证实了无细胞毒性。β-胡萝卜素的生物可及性受油凝胶结构修饰的影响，STOs的生物可及性值为4.0±0.7%，BWOs为2.6±1.1%。结果强调需要优化配方以提高生物活性物质的生物利用度，突出了结构化凝胶在调节消化动力学中的作用。
