Yao Chuxin, Tang Jiao, Mo Yangzhi, Zhong Guangcai, Geng Xiaofei, Yi Xin, Zhang Qianyu, Li Jun, Ma Huimin, Zhao Shizhen, Zhang Gan
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; Guangdong-Hong Kong-Macao Joint Laboratory for Environmental Pollution and Control and Guangdong Provincial Key Laboratory of Environmental Protection and Resources Utilization, Guangzhou 510640, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; Guangdong-Hong Kong-Macao Joint Laboratory for Environmental Pollution and Control and Guangdong Provincial Key Laboratory of Environmental Protection and Resources Utilization, Guangzhou 510640, China.
Sci Total Environ. 2024 Dec 20;957:177632. doi: 10.1016/j.scitotenv.2024.177632. Epub 2024 Nov 23.
Polycyclic aromatic compounds (PAC) are common toxics in combustion particles. Numerous studies on health effects of PAC mixtures focused on limited compounds. It's still challenging to quantify complex PAC mixtures in combustion particles. Recently, benzene polycarboxylic acids (BPCAs) method, which involves conversion of PAC mixtures into a few BPCAs, has been used to quantify complex PAC mixtures in particles. In this study, in vitro biossays were used to evaluate the toxicity of extractable organic matter (EOM) in combustion particles. Analysis with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) identified ~1000 molecules, mostly aromatics (84.47 ± 5.32 %), that positively associate with the EOM toxicity (p < 0.05). We further employed BPCAs method to quantify PAC mixtures in the EOM of combustion particles, and observed the toxicity (especially genotoxicity) of EOM linearly increases with the abundance of PAC mixtures (r: 0.68-0.89, p < 0.05), as it is shown by a data set referring to all source types including biomass burning, coal combustion and vehicle exhaust. The genotoxicity of PAC mixtures in EOM of combustion particles was estimated to be 10-13 times that of benzo[a]pyrene at the same mass concentration. Target analysis of 48 PAC was carried out, but a weaker relationship is found for the toxicity of EOM and the abundance of 48 PAC. Taken together, we suggest PAC-derived BPCAs as a fast predictor of the genotoxicity of combustion particles, which could be promising in routine monitoring of PAC pollution in the air.
多环芳烃化合物（PAC）是燃烧颗粒中常见的有毒物质。许多关于PAC混合物健康影响的研究都集中在有限的化合物上。量化燃烧颗粒中的复杂PAC混合物仍然具有挑战性。最近，苯多羧酸（BPCA）方法，即将PAC混合物转化为几种BPCA的方法，已被用于量化颗粒中的复杂PAC混合物。在本研究中，采用体外生物测定法评估燃烧颗粒中可提取有机物（EOM）的毒性。通过傅里叶变换离子回旋共振质谱（FT-ICR MS）分析鉴定出约1000种分子，其中大部分为芳烃（84.47±5.32%），这些分子与EOM毒性呈正相关（p<0.05）。我们进一步采用BPCA方法量化燃烧颗粒EOM中的PAC混合物，并观察到EOM的毒性（尤其是遗传毒性）随着PAC混合物丰度的增加而线性增加（r：0.68-0.89，p<0.05），这一点在包括生物质燃烧、煤炭燃烧和汽车尾气在内的所有来源类型的数据集中都得到了体现。在相同质量浓度下，燃烧颗粒EOM中PAC混合物的遗传毒性估计是苯并[a]芘的10-13倍。对48种PAC进行了目标分析，但发现EOM毒性与48种PAC丰度之间的关系较弱。综上所述，我们建议将源自PAC的BPCA作为燃烧颗粒遗传毒性的快速预测指标，这在空气中PAC污染的常规监测中可能很有前景。
