Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China.
Plant Mol Biol. 2024 Nov 22;114(6):127. doi: 10.1007/s11103-024-01529-7.
Barley (Hordeum vulgare L.) is the fourth largest cereal crop in the world after rice, wheat and maize. Barley yellow mosaic disease (BYMD) is a serious threat to winter barley production. The evolution and mutation of virus strains lead to the breakdown of the resistance of the originally resistant varieties. It is therefore vital to explore new BYMD resistance genes. In this study, a natural population (334 barley varieties or lines) and a double haploid population derived from the cross between Tam407227 and Franklin were used to search for new quantitative trait loci (QTL) for BYMD resistance. Two major QTL on chromosomes 3H and 7H, respectively, were detected from the genome wide association study and validated in the DH population. Among them, The QTL on 3H (qRYM-3H/qTFRYM-3H) was confirmed to be the reported BYMD resistance gene eIF4E by haplotype analysis. And the QTL on 7H (qRYM-7H/qTFRYM-7H) is a novel QTL that has not been reported before. Another QTL on 2H was identified from the DH population. This QTL is more likely the Rmy16 reported previously. These three QTL showed an additive effect on improving BYMD resistance with the average disease scores from 2.45 (all sensitive alleles for these three QTL) to 0.62 (all tolerant alleles for these three QTL). The candidate genes for the novel QTL qRYM-7H/qTFRYM-7H were predicted based on transcriptome sequencing and qPCR analysis.
大麦（Hordeum vulgare L.）是继水稻、小麦和玉米之后世界上第四大谷物作物。大麦黄花叶病（BYMD）是冬大麦生产的严重威胁。病毒株的进化和突变导致原本具有抗性的品种的抗性失效。因此，探索新的 BYMD 抗性基因至关重要。在这项研究中，利用自然群体（334 个大麦品种或系）和 Tam407227 和 Franklin 杂交产生的双单倍体群体，来寻找 BYMD 抗性的新数量性状位点（QTL）。通过全基因组关联研究检测到分别位于 3H 和 7H 染色体上的两个主要 QTL，并在 DH 群体中进行了验证。其中，3H 上的 QTL（qRYM-3H/qTFRYM-3H）通过单倍型分析被确认为已报道的 BYMD 抗性基因 eIF4E。而 7H 上的 QTL（qRYM-7H/qTFRYM-7H）是一个以前未报道过的新 QTL。DH 群体中还鉴定到 2H 上的另一个 QTL。这个 QTL 更有可能是之前报道的 Rmy16。这三个 QTL 对提高 BYMD 抗性有累加效应，平均病情评分从 2.45（这三个 QTL 的所有敏感等位基因）降低到 0.62（这三个 QTL 的所有耐受等位基因）。根据转录组测序和 qPCR 分析，预测了新 QTL qRYM-7H/qTFRYM-7H 的候选基因。
