Hong Yi, Zhou Hui, Zhang Mengna, Zhang Yuhang, Zhu Juan, Lv Chao, Guo Baojian, Wang Feifei, Li Qingliang, Sun Jie, Xu Rugen
Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, China.
China Resources Beer (Holdings) Company Limited, Beijing, China.
Plant Genome. 2025 Mar;18(1):e20557. doi: 10.1002/tpg2.20557.
Winter barley (Hordeum vulgare) production areas in the middle and lower reaches of the Yangtze River are severely threatened by barley yellow mosaic disease, which is caused by Barley yellow mosaic virus and Barley mild mosaic virus. Improving barley disease resistance in breeding programs requires knowledge of genetic loci in germplasm resources. In this study, bulked segregant analysis (BSA) identified a novel major quantitative trait loci (QTL) QRym.ZN1-7H for barley yellow mosaic disease resistance in an F population derived from the cross between "Nongke 1-6" (H. vulgare) and "Zaoshu 3" (H. vulgare). This QTL, originating from bulbous barley (Hordeum bulbosum), demonstrated stability and was further validated in another F population derived from the cross between "Nongke 2-6" (H. vulgare) and "Supi 1" (H. vulgare). QRym.ZN1-7H accounted for 10.61%-19.34% of the phenotypic variance. The QTL was further fine mapped to the 14- to 39-Mb interval on barley chromosome 7H. Transcriptome analysis identified 53 and 35 differentially expressed genes in roots and leaves (at QRym.ZN1-7H locus), respectively, with nine genes differentially expressing in both tissues. HORVU.MOREX.r3.7HG0650990, a member of the disease resistance protein family (NBS-LRR class), is the most likely candidate gene for QRym.ZN1-7H. Enrichment analysis indicated that QRym.ZN1-7H may be involved in signal transduction in plant innate immune response. This study laid a foundation for barley disease resistance breeding.
长江中下游地区的冬大麦（Hordeum vulgare）产区受到大麦黄花叶病的严重威胁，该病由大麦黄花叶病毒和大麦轻花叶病毒引起。在育种计划中提高大麦抗病性需要了解种质资源中的遗传位点。在本研究中，通过混合分组分析法（BSA）在由“农科1-6”（H. vulgare）与“早熟3号”（H. vulgare）杂交产生的F群体中鉴定出一个新的抗大麦黄花叶病的主要数量性状位点（QTL）QRym.ZN1-7H。该QTL源自球茎大麦（Hordeum bulbosum），表现出稳定性，并在由“农科2-6”（H. vulgare）与“苏啤1号”（H. vulgare）杂交产生的另一个F群体中得到进一步验证。QRym.ZN1-7H解释了10.61%-19.34%的表型变异。该QTL被进一步精细定位到大麦7H染色体上14至39兆碱基的区间。转录组分析分别在根和叶（在QRym.ZN1-7H位点）中鉴定出53个和35个差异表达基因，其中9个基因在两个组织中均差异表达。抗病蛋白家族（NBS-LRR类）成员HORVU.MOREX.r3.7HG0650990是QRym.ZN1-7H最有可能的候选基因。富集分析表明，QRym.ZN1-7H可能参与植物先天免疫反应中的信号转导。本研究为大麦抗病育种奠定了基础。
