转谷氨酰胺酶 2 在促进特发性牙龈纤维瘤病中大连接蛋白合成中的作用。
Role of transglutaminase 2 in promoting biglycan synthesis in idiopathic gingival fibromatosis.
机构信息
Department of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan.
Department of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai, Japan.
出版信息
BMC Oral Health. 2024 Nov 21;24(1):1422. doi: 10.1186/s12903-024-05211-8.
BACKGROUND
This study aimed to elucidate the pathogenesis of idiopathic gingival fibromatosis (IGF).
METHODS
Human gingival fibroblasts (hGFs) were isolated from patients with IGF and periodontitis. Differential gene expression in the hGFs was analyzed using RNA sequencing. Extracellular matrix-related gene expression in the hGFs was analyzed. The effect of specific protein (SP)1 inhibitor or recombinant human transglutaminase 2 (rh-TGM2) on biglycan (BGN) expression in hGFs was also determined.
RESULTS
RNA sequencing showed that TGM2 expression was downregulated and BGN mRNA expression was upregulated in patients with IGF relative to periodontitis. rh-TGM2 stimulation of hGFs in patients with IGF significantly reduced BGN expression. SP1 inhibitors downregulated BGN expression in the hGFs.
CONCLUSION
BGN upregulation via SP1 causes TGM2 downregulation in gingival fibroblasts in IGF.
背景
本研究旨在阐明特发性牙龈纤维瘤病（IGF）的发病机制。
方法
从 IGF 和牙周炎患者中分离出人牙龈成纤维细胞（hGF）。使用 RNA 测序分析 hGF 中的差异基因表达。分析 hGF 中细胞外基质相关基因的表达。还确定了特异性蛋白（SP）1 抑制剂或重组人转谷氨酰胺酶 2（rh-TGM2）对 hGF 中 biglycan（BGN）表达的影响。
结果
RNA 测序显示，与牙周炎相比，IGF 患者的 TGM2 表达下调，BGN mRNA 表达上调。rh-TGM2 刺激 IGF 患者的 hGF 可显著降低 BGN 表达。SP1 抑制剂下调 hGF 中的 BGN 表达。
结论
通过 SP1 引起的 BGN 上调导致 IGF 中牙龈成纤维细胞中的 TGM2 下调。
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